Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVII
Written by Krasimira TASHEVA, Georgina KOSTURKOVA
Rhodiola rosea L. is an endangered medicinal plant due to over harvesting in Bulgaria and in other European countries. The root and rhizomes are rich in pharmacologically and therapeutically active substances like flavonoids, phenolic acids that make the plant of commercial importance. The seed propagation in nature is very poor as far as only of 2 to 30 % of the seeds germinate depending on the ecologically geographic conditions. Thus, in vitro techniques are suitable for propagation of this species. In vitro propagation is possible by direct and indirect organogenesis. The latter is much more difficult in many species, including golden root. The present study aimed to understand the conditions, which provoke undifferentiated tissue initiation and development of shoots. The effects of different plant growth regulators in various concentrations oncallus induction and indirect plant regeneration were investigated. Regenerative callus was received on Murashige and Skoog medium containing 2,4- Dichlorophenoxyacetic acid (0.1 mg Г1)within 28 days. The adventitious buds were differentiated when the calli were subcultured on Murashige and Skoog (1962) medium supplemented with 6-benzylaminopurine (1.0 mg Г1) and indole-3-acetic acid (0.1 mg Г1) within 4 - 5 weeks. Half-strength solid MS with indole-3-butyric acid (2.0mg Г1)and IAA (0.2 mg Г1) exhibited the best in vitro rooting. These results contribute to the understanding of processes of growth and development in vitro of Rhodiola rosea and possibilities for selected of valuable clones and establishment of propagation schemes.
[Read full article] [Citation]
calli
