Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XIX
Written by Ana Raluca BIŢICĂ, Aurel GIURA
In the last decade, the use of double haploid lines in maize breeding programmes has become a standard procedure. This has become possible due to the substantial progress achieved by using in vivo maternal haploid technology induction. Currently, there are in use haploid inducers with inducing efficiency up to 15 %, making possible utilization of double haploid (DH) technology on a large scale. The most important advantages of this system is to shorten the period for obtaining homozygous lines (only 2 years) as compared to the conventional system (5-7 years) and high recognition precision of the putative haploid kernels. The anthocyanin coloration of the aleurone can vary from a small patch to covering the entire aleurone zone, exception kernel basis. Intensity of anthocyanin can range from very poor to strong anthocyanin both in the embryo and aleurone . According to the literature, haploids obtained from dent genotypes are more easily recognized and both anthocyanin coloration and intensity of the coloration are better expressed than in flint genotypes. Since 2013, at NARDI Fundulea, we made some observation regarding the size and intensity of the anthocyanin coloration for the dent and flint genotypes studied. Annotations done on both aleurone and embryo were assessed with scores from 0-4: 0 for the lack of pigmentation and 4 for intense pigmentation. The objective of the present study was to see what score corresponds to a good choice of the haploid kernels.
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anthocyanin coloration
