Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVIII
Written by Dorica BOTAU, Vanda BOLDA, Sorina POPESCU, Oana BOLDURA
The tissue lines derived from the Arieșeni, Retezat and Valea Sebeșului bilberry genotypes grown on a WPM medium supplemented with 40, 60 and 80 mg/l AS, were subject to molecular analysis through amplification of DNA samples with ISSR and RAPD primers. The results of agarose gel electrophoresis of the amplification products show the existence of significant differences in the model of polymorphic bands obtained from tissue lines, compared to the mother plant. Based on the obtained results, the presence of somatic variability, induced in the tissue lines under the influence of AS, is emphasized. It consists in the absence of binding sites of the HB-12 ISSR marker compared to the mother plant. Considering the statements in the specialty literature, concerning the mutagenic effect of growth, we can state that AS caused changes within the DNA level in the bilberry calluses selected by us under the experimental conditions.
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bilberry tissue lines
