Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Luminiţa Raluca SIMIONESCU, Narcisa BĂBEANU, Călina Petruţa CORNEA
The paper aimed to present a review on the social and economic aspects of NBTs, the studies on two different species of plants, subjects of NBT's. The plants variants generated by NBTs are more readily accepted in the market and for crop improvement. In this article we will present briefly the benefits, application and expected developments, regulatory status of NBTs in and outside the EU. It was developed a system for the detection of a broad spectrum of GMOs for analysis of food/feed matrices by the characterization of transgene flanking regions and the typical combinations for transgene constructs. We will describe two different species of plants, subjects of NBTs: 1) Tomatoes for carotenoid sequestration mechanisms and the carotenoid biosynthesis. The carotenoid accumulation and changes in carotenoid profiles suggest that the plastid can adapt to changes in carotenoid content through plastid differentiation and preferential sequestration; 2) Edited maize genome by biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. As a conclusion, CRISPR/Cas9 is the most used technology for genome editing due to its simplicity and efficiency. In this article, we aim to highlight the application of CRISPR/Cas9 technique system, like the powerful genome editing tool for crop improvement.
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