Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVIII
Written by Krasimira TASHEVA, Georgina KOSTURKOVA
Rhodiola rosea is a medicinal plant, containing a range of antioxidant compounds, including p-tyrosol, organic acids(gallic acid, caffeic acid, and chlorogenic acid), and flavonoids (catechins and proanthocyanidins). The stimulating andadaptogenic properties of Rhodiola rosea are attributed to p-tyrosol, salidroside, rhodioniside, rhodiolin, rosin,rosavin, rosarin, and rosiridin. The recent investigations showed that sucrose influences secondary metabolism in cell,tissue and organogenic cultures. Higher sucrose levels in certain limits lead to increased production of alkaloids in invitro cultures of various plants. The aim of this work was to study the influence of different sucrose concentrations onthe process of callus formation. The nutrient medium containing different percentage of sucrose (20, 30 and 40 %) wereused. Leaves excised from in vitro propagated golden root plants were plated for callus induction and maintenance. Theresults showed that the callogenesis of golden root plants was more effective (2 - 3 folds) when sucrose was 20 g/lcompared to higher concentrations of sucrose at the background of the same combinations of plant growth regulators.The obtained calli differed in structure and color. HPLC analysis of the calli was performed.
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