Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XIX
Written by Lidiya GEORGIEVA, Ivan IVANOV, Andrey MARCHEV, Ina ANEVA, Vasil GEORGIEV, Panteley DENEV, Atanas PAVLOV
The aim of the study was development of protocols for obtaining callus cultures from Fumaria rostellata Knaf. For the purpose leaves, stems and flowers of the investigated plant were used as explants. Callus was initiated on a Murashige and Skoog nutrient medium, supplemented with 30 g/l sucrose, 5.5 g/l “Plant” agar and various concentrations (0.2; 0.5; 1.0; 2.0; 3.0 mg/l) of auxin (2,4-dichlorophenoxyacetic acid) and cytokine (6-benzylaminopurine). The best results for initiation of calli were observed at a low concentration of 2,4-D (0.2 mg/l or 0.5 mg/l) and a high concentration of BAP (2.0 mg/l or 3.0 mg/l). It is well known that Fumaria plants are rich sources of isoquinoline alkaloids and obtained in vitro cultures from F. rostellata could be used as potential technological matrixes for development of a commercial process for protopine production.
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2,4-D
