Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Georgiana MICU, Oana-Alina BOIU-SICUIA, Călina Petruţa CORNEA
An important enzyme for both the pharmaceutical and food industries is L-asparaginase (EC 3.5.1.1). This enzyme is produced by a wide variety of microorganisms. However, their potential use as sources of L-asparaginase at industrial scale is limited if glutaminase and urease are also produced. This is mainly due to the complexity and expenses of the purification process required to obtain l-asparaginase which make the production system inefficient. In order to select L-asparaginase highly producing strains, lacking detectable glutaminase and urease activity different isolation steps were established and rapid tests are the first recommended. However such studies need to be completed with quantitative analysis. Moreover, additional molecular studies can also confer useful information regarding the biotechnological potential of the selected strains. The aim of this paper is to correlate the microbiologic and biochemical tests with genetic approaches in order to improve the selection process of biotechnologically relevant Bacillus strains.
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