Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Ana Raluca COJOACĂ(BIȚICĂ)

Accelerating the development of homozygous lines and consequently hybrids is an important aspect of the maize breeding programs. Doubled haploid technology has successfully replaced the traditional method of obtaining homozygous lines in maize breeding programs in Europe, North America, China and Central America, due to the clear advantages in terms of timing and important reduction of the workload and costs by eliminating controlled pollination and relatively simple methodology. Haploid inducing efficiency is influenced by the genotype of the population submitted to the induction, haploid inducing capacity of the inducer and the inducing protocol (time and type of pollination). The study was carried out in special climatic conditions of the year 2016 (the absolute maximum temperature was 35.80C for June and 35.70C for July. The results showed that the source genotype used in the induction nursery influenced the anthocyanin coloration in both aleurone and embryo. Clear variability regarding the anthocyanin coloration was observed among the ears from the same genotype and even between aleurone and embryo from the same ear. Comparing the PHK (putative haploid kernels) number from the top and bottom of the ear, the top average is distinct significantly positively over the bottom average PHK number. Similarly to controlled pollination, the highest percentage of PHK remains on top of the ear in the case of this experiment with open pollination induction nursery.

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