Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Irinel Gabriel PROCA, Florentina MATEI, Camelia Filofteia DIGUȚĂ, Ștefana JURCOANE

Saline waters are environments largely unexplored in which organisms are able to survive/withstand in the extreme conditions. The aim of this research was to explore saline water located in Lopătari, Buzău County, România. Several physico-chemical properties were assessed such as pH, density, concentration of cations and anions. The pH of water sample was slightly acidic (5.2).The ions of sodium, chloride and total dissolved solids were detected dominant 129.9 g.L^-1, 209.83 g.L^-1 and 113 gL^-1, respectively. A total of fourty four (44) aerobic bacteria were isolated on culture media supplemented with 5%, 10 %, and 15% NaCl, respectively. The isolates were screened by morphological criteria, Gram stain. All bacterial isolates were tested for their tolerance to different concentrations of salt in solid media. Two bacterial isolates (4.5%) has grown over a wide range of salt concentrations ranging from 0% to 12.5%, 17 bacterial isolates (38.6%) under salt concentration between 0% and 7.5%. Other 19 bacterial isolates (41%) demonstrated a large salt tolerance ranging from 5 to 25%. Halophiles can offer important opportunities in biotechnological applications such as food, pharmaceutical, detergents, environmental bioremediation and biosynthetic processes, being one of the main reasons for the future research of them.

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