Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXII
Written by Ivaylo SIRAKOV, Katya VELICHKOVA, Desislava SLAVCHEVA-SIRAKOVA

In current study the antibacterial activity of different medicinal herbs against fish pathogen Aeromonas hydrophila was evaluated. The water extract of medicinal herbs (Glycyrrhiza glabra, Rhodiola rosea, Althaea officinalis, Sambucus nigra, Inula helenium, Pinus sylvestris, Ocimum basilicum, Salvia officinalis) were prepared by the following method: the plants were extracted in water solution at proportion 1:10. The received homogeny solutions were filtered and centrifuged at 7000 rpm for 30 minutes. Afterwards the extracts were filtered with sterile syringe filters with the size 0.2 μm. The bacterial strain of Aeromonas hydrophila (АТСС 7965) was used in current research. The bacterial activity of plant extract against Aeromonas hydrophila were tested with disk diffusion method. The highest antibacterial effect against Aeromonas hydrophila were determined in water extract of Salvia officinalis and its inhibition zone was higher with 33.34% compared with the size of zone measured for the control variant.

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