Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXII
Written by Mihaela DUMITRU, Cristina TABUC, Ionuț SORESCU, Andreea VASILACHI, Mihaela HĂBEANU, Stelian PETRE, Ștefana JURCOANE

The objective of this experimental study was to evaluate the enzymatic hydrolysis process in order to obtain fermentable sugars from feed materials (maize, field peas, field beans), usually used in animal nutrition. For breaking down the carbohydrates from cell materials raw, it was used a carbohydrase enzyme preparation (IUB No. 3.2.1.6) with activity of 50 FBG/g. The enzyme was tested at concentration of 0.05-0.5% (w/v). For measuring accumulation of reducing sugars, it was used 3,5 dinitrosalicylic acid (DNS) assay. Results from experimental samples supplemented with carbohydrase enzyme were compared with control sample without enzyme addition. The activity of carbohydrase enzyme was evaluated using a carboxymethyl cellulose medium (4.19 UDNS/ml). For the experimental data, the total amount of available sugars was significantly higher (P<0.0001) for peas (19.60±0.105), followed by maize (17.17±0.105) and beans field (10.50±0.055) at a concentration of 0.5% enzyme. The results suggest that hydrolytic potential of enzyme preparation to produce fermentable sugars was superior on field peas, knowed as source of protein in animal nutrition and can be used as feed additive to improve nutrient availability.

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