Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Andreea TICAN, Mihaela CIOLOCA, Nicoleta CHIRU, Carmen BĂDĂRĂU
Necessity of finding genotypes adapted to drought has become urgent due to the effect of this type of stress on potato production. The most important phase, indispensable for improving drought tolerance is to identify genotypes tolerant and sensitive to drought. In this study, to induce in vitro water stress were used polyethylene glycol and sorbitol that was comparable with the basic medium MS, considered control. Determinations were performed 4 weeks after inoculation of mini cuttings belonging to five varieties of plantlets (Ruxandra, Sarmis, Gared, Marvis, Rustic) and the parameters analyzed were next: number of leaves, number of internodes, height of plantlets, root length, weight of fresh plantlet, weight of fresh root. Medium in which was added PEG with different concentrations significantly reduced the average weight of fresh plantlet and root compared with the control medium and medium with sorbitol and significantly reduced the mean number of internodes, the average height of the plantlet, the average root length. This osmotic agent (PEG) can be recommended for in vitro simulation of drought to identify tolerant genotypes to hydric stress.
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