Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Cristina Valentina ALBU (PROCA), Raluca Ştefania ENCEA, Camelia DIGUŢĂ, Florentina MATEI, Călina Petruţa CORNEA
Laccase belongs to the blue multicopper oxidases and participates in degradation of polymers, ring cleavage of aromatic compounds and cross-linking of monomers. It is distributed in higher plants and fungi. It is present in Ascomycetes, Deuteromycete, and Basidiomycetes and abundant in lignin-degrading white-rot fungi. Lacasse has been reported to be produced by different mushrooms (Trametes, Ganoderma, Pleurotus) and by filamentous bacteria (Streptomyces) or fungi (Aspergillus). The article proposes a comparative analysis of the optimal conditions for laccase production in the case of macromycetes and some micromycetes, like fungi or filamentous bacteria, meanwhile describing the isolation, purification and characterization of the laccase produced by such organisms. All these issues will be approached through the biotechnological application of these enzymes (dye decoloration, bioremediation etc).
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