ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364
     

    COMPARATIVE ANALYSIS OF Artemisia annua EXTRACTS OBTAINED BY MODERN AND CLASSIC EXTRACTION TECHNIQUES

    Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
    Written by Corina BUBUEANU, Iulian TECU; Denis NEGREA; Ioana TABREA; Adela STARAS; Ionica BAJENARU

    The aim of our study was to assess the artemisinin and phenolic compounds content of extracts obtained from Artemisia annua by classic solvent extraction comparatively with the extract obtained by microwave. For the classic technique, the solvents used were water, methanol, ethanol, and acetone. For microwave extraction, the solvent was ethanol 90% (v/v). The phenolic compounds were identified by HPTLC and artemisinin was also quantified by HPLC. In all samples rutin, hyperoside, chlorogenic and cafeic acids were identified. The highest quantity of artemisinin was found in extracts obtained by microwave extraction. The highest DPPH radical scavenging was obtained for aqueous extract, whereas methanolic extract exhibited almost a constant activity in different concentrations. The results obtained for total antioxidant capacity by the phosphomolibdenum method were similar to the DPPH method.

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