ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364
 

ASSESSMENT OF GENETIC SIMILARITY AND PURITY DEGREE AMONG SEVERAL ROMANIAN MAIZE INBRED LINES USING SSR MARKERS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Valentina VASILE, Matilda CIUCĂ, Elena NICOLAE, Cătălina VOAIDEȘ, Călina Petruța CORNEA

Maize (Zea mays ssp. mays) is today one of the most important cereal crops used not only for human consumption but although for feed or for industrial purposes, without the genetic evolution and the active intervention of breeders in the plant constant improvement, maize would not have today significance. In this study, seeds from thirteen maize inbred lines (LC1-LC13) were analysed using eight SSRs markers recommended for seed varietal purity assessment. The seeds classes for maize inbred lines used in this study were pre-basic and basic seeds. Three of the maize inbred lines chosen for testing from both pre-basic and basic categories were analysed in order to verify that the varietal purity is preserved. High genetic similarity was between inbred line LC1 and LC2. SSR marker phi015 was the most polymorphic marker followed by umc1545, umc1448 and umc1117. The SSRs markers that showed low polymorphism were umc1061 and phi109275. The aim of this study was to select the most informative SSRs markers which fit to prove the varietal purity and to assess genetic diversity for maize seeds.

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© 2019 Scientific Bulletin Series F.“Biotechnologies“. All Rights Reserved. To be cited: Scientific Bulletin Series F.“Biotechnologies“.

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