Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Grigore-Mihăiță STAN, Diana CONSTANTINESCU-ARUXANDEI, Dan Florin MIHĂILESCU, Florin OANCEA
The paper aimed to identify in silico bioactive peptides with antioxidant and antihypertensive effects from silver carp collagen. This approach involved the use of a wide range of specialized online databases and tools to identify bioactive peptides from various protein sources. In this case, the collagen type-I alpha-1 protein sequence was extracted from UniProtKB with the identification code A0A077B3P8. The digestion was simulated using the BIOPEP database with the following enzymes: subtilisin, papain and pepsin. ExPASy ProtParam, Peptide Ranker, PepCalc, and ToxinPred showed that silver carp collagen is a significant source of biologically active peptides, health promoters with potential antihypertensive and antioxidant effects. The computational approach used in this study offered useful initial insights for more extensive studies.
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