Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Giancarla VELICEVICI, Emilian MADOŞĂ, Dorin CAMEN, Sorin CIULCA, Adriana CIULCA, Carmen BEINȘAN, Mihaela MĂLĂESCU, Iuliana CREȚESCU
An important tool in fruit tree breeding programs is genetic variation. In present work were studied the genetic diversity of some pears genotypes from West part of Romania. For this way we used 10 randomly amplified polymorphic DNA (RAPD) primers. Five out of the 10 primers used in this study amplified clear and reproducible bands. The RAPD primers produced 62 bands, and 46 of them were polymorphic, with an average of 12.4 amplicons/primer. The polymorphic bands, registered per primer ranged from 6 (OPA18) to 10 (primer OPA12). Total polymorphism generated by a certain primer (PIC), registered values between 0.39 P11 0.45 OPA18. The discrimination index (PI), presented values among 2.72 for the primer OPA18 and 5.38 for P-25 primer, which had the highest capacity to generate polymorphic bands to genotypes being studied. Results showed the suitability of RAPD analysis in genetic diversity studied of pear landraces.
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