Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Oana GHERGHESCU, Angela CĂŞĂRICĂ, Mișu MOSCOVICI, Florentina ISRAEL-ROMING
Lipases have gained attention worldwide, due to their potential for diverse applications and their stability and selectivity. The study’s aim was to identify microorganisms with the ability to synthesize extracellular lipase. In order to achieve this, 20 microbial strains were cultivated on selective solid agar media and lipase producing microorganisms were selected based upon their ratio of the diameter of the halo (if present) and the colony’s diameter. The bacterial strains were cultivated on three different selective media, the results indicating that the best screening medium was TBA and the strain that was estimated to have the highest lipase activity was Bacillus subtilis ICCF 20. Yeast strains were cultivated on two selective media and M4D was selected as the best screening medium. The highest producer of lipases was considered to be Yarrowia lipolytica ATCC 16618 ICCF 214. The fungal strains were cultivated on two selective media and the best screening medium was determined to be YS. Two fungal strains were selected as having the highest lipase activity: Aspergillus niger (P4 C36) ICCF 24 and Aspergillus awamory (P2 C114) ICCF 259.
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