Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Agnes TOMA, Oana CRACIUNESCU, Rodica TATIA, Vasile Sorin MANOIU, Florentina ISRAEL-ROMING, Gabriela LUTA, Daniela BALAN, Ştefana JURCOANE
In the present study, novel biomaterials based on mixtures of collagen and peptides isolated from aquaculture fish residues and marine chitosan solution at pH 5 were prepared, conditioned as 3D porous sponges and characterized to select best variant for biomedical applications. The physico-chemical properties of the biomaterials were investigated by determination of their in vitro biodegradability, swelling degree and porosity, while morphology was observed by scanning electron microscopy (SEM). In addition, the biocompatibility of the prepared biomaterials was assessed by cell viability of L929 fibroblast cultures cultivated in the presence of the prepared sponges by MTT assay. The results indicated that the biomaterials prepared from mixtures of collagen and chitosan at pH 5 and mixtures of collagen, chitosan at pH 5 and peptides had lower in vitro biodegradability in the presence of collagenase, higher degree of swelling and increased porosity, compared to a sponge of fish collagen (control). The cell viability values recorded in L929 fibroblast culture cultivated in the presence of the prepared biomaterials were high, indicating a good biocompatibility with skin specific cells. In conclusion, this study demonstrated that the biomaterials prepared from fish collagen and marine chitosan at pH 5 with or without addition of fish peptides have optimal physico-chemical and structural properties and good biocompatibility, recommending their further testing and use in skin tissue engineering.
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