Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Giancarla VELICEVICI, Emilian MADOŞĂ, Dorin CAMEN, Sorin CIULCA, Adriana CIULCA, Carmen BEINȘAN, Mihaela MĂLĂESCU, Iuliana CREȚESCU

An important tool in fruit tree breeding programs is genetic variation. In present work were studied the genetic diversity of some pears genotypes from West part of Romania. For this way we used 10 randomly amplified polymorphic DNA (RAPD) primers. Five out of the 10 primers used in this study amplified clear and reproducible bands. The RAPD primers produced 62 bands, and 46 of them were polymorphic, with an average of 12.4 amplicons/primer. The polymorphic bands, registered per primer ranged from 6 (OPA18) to 10 (primer OPA12). Total polymorphism generated by a certain primer (PIC), registered values between 0.39 P11 0.45 OPA18. The discrimination index (PI), presented values among 2.72 for the primer OPA18 and 5.38 for P-25 primer, which had the highest capacity to generate polymorphic bands to genotypes being studied. Results showed the suitability of RAPD analysis in genetic diversity studied of pear landraces.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Mihaela GEICU-CRISTEA, Elisabeta Elena POPA, Paul-Alexandru POPESCU, Mihaela Cristina DRĂGHICI, Amalia Carmen MITELUȚ, Mona Elena POPA

Producing safe and high-quality fish and seafood products, for both domestic and export markets must be considered a priority for the entire fish and seafood chain, from fishers and producers towards consumers and food safety competent national authorities, who should update the relevant food safety legislation and ensure compliance with it. The aim of this study is to highlight the rules of hygiene and food safety that are imposed on the fisheries and seafood sector in order to prevent staff illness with Covid-19 and ensure the safety of products. Several practical recommendations are given for completion and improvement of the current preventive measures such as good hygiene practices to which is added specific protocols to safeguard the health of the employees who works in the fish and seafood production and processing sector.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Mohammed Shaymaa Omar MOHAMMED, Narcisa BABEANU, Călina Petruţa CORNEA, Nicoleta RADU

Limonene is a biomolecule that can be easily obtained from plant sources with remarkable biological effects. It is found in large quantities in citrus essential oils (concentrations up to 98%) and in moderate amounts in essential oils obtained from various species of geranium. The article presents the reasons why this compound may have potential uses in regenerative medicine, especially in dermatological applications, where these compounds or formulations which contain this compound, alone or via intermediary products which result from metabolization (such as perillyl alcohol) can accelerate wound healing, inhibit skin tumors development, or inhibit pathogenic microorganisms such as Staphylococcus aureus or Pseudomonas aeruginosa. Studies performed in vivo, on healthy persons who have tested in time the skin cleaning products which contain limonene or essential oils with limonene, were confirming that the products with Limonene do not give perturbation to normal skin microbiota. These data recommend Limonene as a potential candidate for regenerative medicine applications in the field of dermatology.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Daniel COJANU, Maria Cristina LUMÎNARE, Monica Mihaela DINU, Ana-Cristina FĂTU

Using two different bioassay methods, the pathogenicity of three isolates of Beauveria bassiana, one of B. pseudobassiana and one of Metarhizium anisopliae, was evaluated against two model insects, Plodia interpunctella and Galleria mellonella in larval stage. In laboratory conditions, the insects were treated by immersion in a conidial suspension of 1x10₈ UFC/ml and by dusting. Larval mortalities were recorded daily, 14 days post-exposure. All the fungal isolates have been shown to be pathogenic to test insects. Thus, two isolates of B. bassiana determined the highest mycosis percentages for test insects both by immersion and dusting. One B. bassiana isolate (BbTd1) killed P. interpunctella larvae in the shortest time (6 days), and the other isolate (BbTd2) determined the highest mycoses percentage, followed by B. pseudobassiana isolate (BpPa) by both treatment methods. The highest mycosis percentage was determined by BbTd2 and BPa isolates in the dusting treatment. G. mellonella larvae proved to be the least sensitive to fungal treatments applied by immersion (MST>50%). In the dusting treatment, the BbTd1 and MaF isolates induced the highest percentage of mycosis of G. mellonella larvae. All isolates have pathogenicity against test insects, indicating their possible use for biocontrol.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Ionuț-Cristian RUSU, Alexandru Valentin ZĂGREAN, Florentina ISRAEL-ROMING

Macromycetes are a topic of great interest for researchers around the globe and in our country, mushrooms being well-known for their nutritional, gourmet and medicinal values. In line with current ecological trends, a sustainable solution for environmental protection is to produce mushrooms by bioconversion of some lignocellulosic waste/by-products of agro-forestry origin and therefore to examine the qualitative and quantitative impacts of multiple substrate recipes upon mushroom production. Assiduous research has improved the biotechnologies for the production and propagation of mycelium used for seeding spawn, in parallel with obtaining and characterization of extracts rich in bioactive compounds from both mycelium and fruiting bodies of edible and medicinal species of macromycetes. This review proposes a current presentation of the knowledge at the intersection of these research directions, focusing on their applications, targeting the species of the genus Pleurotus.

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GENETIC DIVERSITY AMONG SOME PEARS GENOTYPES FROM WEST PART OF ROMANIA ON THE BASE OF RAPD MARKERS

Written by Giancarla VELICEVICI, Emilian MADOŞĂ, Dorin CAMEN, Sorin CIULCA, Adriana CIULCA, Carmen BEINȘAN, Mihaela MĂLĂESCU, Iuliana CREȚESCU
Pages 9 to 13

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PATHOGENICITY OF Beauveria bassiana, B. pseudobassiana, AND Metarhizium anisopliae INDIGENOUS ISOLATES AGAINST Plodia interpunctella AND Galleria mellonella IN LABORATORY ASSAYS

Written by Daniel COJANU, Maria Cristina LUMÎNARE, Monica Mihaela DINU, Ana-Cristina FĂTU
Pages 14 to 22

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STIMULATION OF SEABUCKTHORN (Elaeagnus rhamnoides) MICROBIAL SYMBIOSES

Written by Ion-Mihai GIURESCU, Călina Petruța CORNEA, Florin OANCEA
Pages 23 to 30

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RESEARCH ON BIOCONVERSION OF LIGNOCELLULOSIC WASTE FOR THE CULTIVATION OF BIOCOMPOUNDS PRODUCING MACROMYCETES

Written by Ionuț-Cristian RUSU, Alexandru Valentin ZĂGREAN, Florentina ISRAEL-ROMING
Pages 31 to 40

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BIOSYNTHESIS OF INULINASES BY Aspergillus terreus USING ORANGE PEELS POWDER AS A POTENTIAL SUBSTRATE

Written by Maria Monica PETRESCU, Dana Maria MIU, Gabriela SAVOIU, Mihaela Carmen EREMIA
Pages 41 to 46

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EVALUATION OF THE IMPACT OF THE Trichoderma TREATMENT IN BUZĂU BELL PEPPER (Capsicum annuum) CULTURE 10

Written by Cristian-Alexandru TOADER, Floarea BURNICHI, Dumitru-Mitel TOMA, Vasilica MANEA, Emilian MIREA, Mona Elena POPA
Pages 47 to 51

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COMPARISON OF DIFFERENT TYPES OF MOLECULAR MARKERS USED IN GENETIC DIVERSITY STUDIES OF BROOMRAPE FROM SERBIA

Written by Maria DUCA, Ana MUTU, Ina BIVOL
Pages 52 to 59

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THE ANTIOXIDANT EFFECT OF RAW BEE POLLEN COLLECTED FROM ECOLOGICAL CROPS - MINIREVIEW

Written by Mirela DRĂGAN, Roxana SPULBER, Oana LIVADARIU, Ștefana JURCOANE
Pages 60 to 67

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BIOTECHNOLOGICAL APPLICATIONS OF MYCORRHIZAL PRODUCTS IN INTENSIVE AGRICULTURE

Written by Marina DRAGOTA, Ştefana JURCOANE, Virgil UNTARU
Pages 68 to 72

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USE OF HYDROGELS AS A SUSTAINABLE SOLUTION FOR WATER AND NUTRIENTS MANAGEMENT IN PLANT CULTURE

Written by Amalia-Carmen MITELUȚ, Silvana Mihaela DĂNĂILĂ-GUIDEA, Paul-Alexandru POPESCU, Mihaela-Cristina DRĂGHICI, Elisabeta Elena POPA, Mona Elena POPA
Pages 73 to 84

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USE OF MICROENCAPSULATION AND NANOENCAPSULATION TECHNIQUES IN DAIRY TECHNOLOGY

Written by Ece BÜYÜKGÜMÜŞ, Merve ÖZCAN, Selda BULCA
Pages 85 to 91

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CHARACTERIZATION OF FEED CONTAMINATION BY Fusarium sp. - A REVIEW

Written by Asawir Esamaldeen Ebrahim MOHAMED, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA
Pages 92 to 103

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CHARACTERIZATION OF FEED CONTAMINATION BY Fusarium sp. - A REVIEW

Written by Asawir Esamaldeen Ebrahim MOHAMED, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA
Pages 104 to 109

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IMPACT OF COVID-19 PANDEMIC ON THE FOOD SAFETY REQUIREMENTS IN THE FISH AND SEAFOOD CHAIN

Written by Mihaela GEICU-CRISTEA, Elisabeta Elena POPA, Paul-Alexandru POPESCU, Mihaela Cristina DRĂGHICI, Amalia Carmen MITELUȚ, Mona Elena POPA
Pages 110 to 115

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AMINO ACIDS COMPOSITION OF WHEAT-GERMINATED LEGUMES COMPOSITE FLOURS

Written by Florin URSACHI, Denisa ATUDOREI, Mădălina UNGUREANU-IUGA, Georgiana Gabriela CODINĂ
Pages 116 to 124

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CURRENT STATUS OF THE APPLICATIONS OF PULLULAN AND ITS DERIVATIVES IN BIOMEDICAL FIELD

Written by Fawzia SHAAT, Ramona-Daniela PAVALOIU, Cristina HLEVCA
Pages 125 to 132

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GENETIC APPROACHES TO SELECT L-ASPARAGINASE PRODUCING Bacillus STRAINS

Written by Georgiana MICU, Oana-Alina BOIU-SICUIA, Călina Petruţa CORNEA
Pages 133 to 138

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LIMONENE - A BIOMOLECULE WITH POTENTIAL APPLICATIONS IN REGENERATIVE MEDICINE

Written by Mohammed Shaymaa Omar MOHAMMED, Narcisa BABEANU, Călina Petruţa CORNEA, Nicoleta RADU
Pages 139 to 150

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SCREENING OF CULTIVATION MEDIA FOR LDPE BIODEGRADATION BY Pseudomonas fluorescens

Written by Olga POSTOLACHI, Inna RASTIMESINA, Valentina VORONA, Vera MAMALIGA
Pages 151 to 157

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BIODEGRADATION OF NOVEL POLYLACTIC ACID BASED BIOMATERIALS BY DIFFERENT METHODS

Written by Elisabeta Elena POPA, Maria RÂPĂ, Mihaela GEICU-CRISTEA, Amalia Carmen MITELUŢ, Paul-Alexandru POPESCU, Mihaela Cristina DRĂGHICI, Silvana Mihaela DĂNĂILĂ-GUIDEA, Mona Elena POPA
Pages 158 to 164

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Olga POSTOLACHI, Inna RASTIMESINA, Valentina VORONA, Vera MAMALIGA

The research aimed to select the optimal mineral salt medium (MSM) for low-density polyethylene (LDPE) biodegradation by the strain Pseudomonas fluorescens CNM-PFB-01. Four culture media were selected, which differed in salt content, N: P and C: N ratio. After 40 days of submerged cultivation, the following parameters were determined: catalase activity, pH of culture media, biomass accumulation, rate of degradation and the tensile tests of LDPE. It was observed that the strain is catalase-negative both in the control without LDPE and in the presence of LDPE. A weak positive reaction was established only on MSM 4 supplemented with LDPE. In the presence of polyethylene, the pH of the media increased, especially on the MSM 4 - by 0.6 units. The addition of polyethylene to the growth media stimulated the bacterial biomass accumulation by 2-3.6 times. The degradation rate of polyethylene films ranged from 0.37% to 0.86% depending on the culture medium. The tensile test showed increased elasticity of the plastic in the variants treated with bacterial strain. In conclusion, in order to stimulate the biodegradation of LDPE by the strain P. fluorescens CNM-PFB-01, the medium MSM 4 (N: P ratio 4.30: 1 and C: N ratio 0.29: 1) was selected.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Ion-Mihai GIURESCU, Călina Petruța CORNEA, Florin OANCEA

This paper reviews the present knowledge regarding the stimulation of the microbial symbioses of the sea buckthorn (Elaeagnus rhamnoides syn. Hippophae rhamnoides). Sea buckthorn is an actinorhizal plant, developing nitrogen-fixing symbioses with the actinobacteria from the Frankia genus. At the same time, sea buckthorn roots can form endomycorrhizal symbioses with various arbuscular mycorrhizal (AM) fungi. AM symbiosis increases nutrient uptake and nutrient use efficiency, especially on phosphorus and micro-elements. Due to these microbial symbioses, sea buckthorn is an efficient colonizer of marginal lands and a suitable crop for low-inputs organic/ecological farming. Helper bacterial strains were demonstrated to promote microbial symbiosis between Frankia actinobacteria or AM fungi with roots of other host plants. Only scarce and indirect information suggests the involvement of gram-positive, endospore-forming bacteria as a helper of microbial symbiosis for sea buckthorn. Also, there is no information regarding the role of rhizosphere signals in promoting the sea buckthorn microbial symbioses. The finding of this paper highlights the need for future works focused on the stimulation of sea buckthorn symbioses.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Mirela DRĂGAN, Roxana SPULBER, Oana LIVADARIU, Ștefana JURCOANE

Bee products containing bee pollen collected from flowers and harvested by humans from the hive, include both active principles produced by plants and active principles added during collection, processing, and storage by bees. Pollen, is one of the most bee products used in apitherapy, is a valuable source of bioactive substances, as it contains most of the active ingredients that are directly assimilable in the human body (vitamins, minerals, hormones and substances acting as prehormones, enzymes and simple carbohydrates) and have by adding in the diet a wide range of indications, recommendations and applications. As a result, this mini-review systematically presents both the highlighting of the influence of pollen in daily human consumption and the analysis of composition, antioxidant activity, quality parameters and sensory properties of biologically active pollen harvested from organically grown honey crops.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Amalia-Carmen MITELUȚ, Silvana Mihaela DĂNĂILĂ-GUIDEA, Paul-Alexandru POPESCU, Mihaela-Cristina DRĂGHICI, Elisabeta Elena POPA, Mona Elena POPA

The current climate changes are felt at global level through two primary consequences, namely the increase of day and night temperatures and the decrease of water availability, a fact that determines an important impact in the practices of cultivating plants. Due to this reason, superabsorbent materials such as hydrogels, have been developed to be used especially in plant cultivation systems. The practical relevance of the use of hydrogels in plant substrate could lead to an improvement of water and nutrients management conditions in the field of agriculture. Hydrogels act as a reservoir of water and/or nutrients with gradual release according to plant requirements and in a controlled manner. Thus, a favorable environment for the development of roots and plants is created and maintained throughout their growth. At the same time, this technology brings advantages to the physical characteristics of the soil, by improving the porosity of the soil, thus considerably reducing the undesirable effects observed when applying conventional watering techniques, such as soil drying after sprinkler irrigation and soil erosion during gravity irrigation.

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