Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Diana GROPOŞILĂ-CONSTANTINESCU, Gabriela MĂRGĂRIT, Gabriela POPA, Dana BARBA

The skin has remarkable properties of protection and defense against external aggressions, so it is very important that its health is permanently maintained. The main purpose of this research paper consisted in the formulation of two moisturizing creams based only on natural ingredients. The main ingredients were: fresh lavender flowers, aloe vera gel and coconut butter. The moisturisers were organoleptically, physicochemically and microbiologically analysed. The organoleptic tests showed that the moisturizing creams homogenized very well, did not show phase separations and the smell was very pleasant, specific to lavender. The pH values, between 5.0 and 5.3, were close to the optimal recommended, 5.5, which means that their use will have positive effects on the skin. Following the determination of the acidity index, values between 1.68 and 1.91 were obtained, which means that the products can be used safely, as they have not undergone alteration processes. Microbiological analyses showed low contamination with bacteria and fungi, as both lavender and aloe vera are known for their antibacterial and antifungal effects.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Ricardo MIR, Antonio CALABUIG-SERNA, Jose María SEGUÍ-SIMARRO

Upon fertilization, plants produce zygote-derived embryos that will develop into new individuals. The study of its regulation is important to overcome the post-zygotic barriers that may prevent hybridization, requiered for the development of new cultivars. The study of zygotic embryo development is challenging, since they are surrounded by a multilayer maternal tissue, and are difficult to reach. Alternatively, plants produce embryos from specific somatic cells under certain in vitro conditions. This process, referred to as somatic embryogenesis, has been established as a model to study plant embryogenesis, due to its similitudes with zygotic embryogenesis and its technical feasibility. Moreover, the production of somatic embryos allows to efficiently propagate plant material. Somatic embryogenesis is regulated by stimuli of different nature. Among others, calcium gradients within cells and tissues are important to achieve a proper consecution of embryogenesis from somatic cells. In this review, we summarize the most relevant advances in different plant species by using multiple approaches that shows the role of calcium in the regulation of somatic embryogenesis.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Ionuț GIURESCU, Tatiana-Eugenia ȘESAN, Stere BADJU, Carmen LUPU, Florin OANCEA

This paper presents a process for preparing from sea buckthorn branches sawdust of a compost by using a multipurpose Trichoderma strain (T. harzianum Td50b). The used strain accelerates the degradation of the lignocellulosic material and has biocontrol and plant biostimulant characteristics. The branches, resulting as a by-product of harvesting sea buckthorn berries and leaves, were grounded and extracted to recover bioactive ingredients - flavonols, flavones, phenolic acids, proanthocyanidins, triterpenoids, hydrolysable tannins, serotonin. The resulting sawdust, depleted in polyphenols with potential anti-fungal activity, was normalized to 90% water activity with ammonium nitrate solution 0.2M, supplemented with 10% eggshells powder, and inoculated with 2% alginates beads granules containing 10₉spores T. harzianum Td50b per gram. After incubation for 50 days at room temperature in aerobic conditions, the survival of Trichoderma was analyzed by cultivation on selective media. The Trichoderma re-isolated from sea buckthorn branch compost maintained its antagonistic and plant biostimulant characteristics.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Ionuț Georgică MARCHIDAN, Mihaela GEICU-CRISTEA, Elisabeta Elena POPA, Gabriela BUTNARIU, Narcisa Elena BĂBEANU

Lavandula spp., commonly known as lavender, is an aromatic and medicinal plant that belongs to the Lamiaceae family and includes 47 known species of flowering plants of which we mention Lavandula angustifolia, Lavandula stoechas, Lavandula pedunculata, Lavandula dentata, Lavandula multifida, Lavandula latifolia, Lavandula viridis, Lavandula lanata, etc., many of them being hybrids or varieties with important economic value for the pharmaceutical, cosmetic, perfumery, food and agricultural industries. This paper reviews recent literature regarding the characteristics and properties of Lavandula spp., providing a comprehensive view about the use of lavender essential oils and plant extracts in various industrial fields. Variability is discussed by species, geographic area, plant material and extraction methods.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Behnaz RAHMANIFAR, Oana LIVADARIU, Călina Petruța CORNEA

Salvia genus is the largest member of the Lamiaceae family with around 900 species and is widely distributed in tropical and temperate regions. These species are usually aromatic and have numerous pharmacological and therapeutic applications. They are used in traditional medicine to treat eczema, colds, bronchitis, digestive problems, sore throat, tuberculosis, haemorrhage, some cardiovascular and menstrual disorder. Modern studies suggest that various Salvia species have antibacterial, antifungal, anticancer, antioxidant, and anti-inflammatory properties. These activities are consequence of biosynthesized bioactive substances, including terpenoids, flavonoids, phenolic compounds, etc. These chemical constituents refer to as secondary metabolites and exert multiple therapeutic activities without showing important side effects. The present review summarized the information published in the scientific literature on the secondary metabolites in Salvia spp. In addition, the recent biotechnological approaches, advances in metabolic engineering strategies, successful results and potential problems were presented.

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COMPARISON OF CLASSICAL VERSUS qPCR METHODS IN THE DETECTION OF Botrytis cinerea IN RASPBERRIES

Written by Quthama AL ZAIDI, Florentina BADEA, Camelia Filofteia DIGUŢĂ, Florentina MATEI
Pages 7 to 11

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SCREENING OF SSR AND EST-SSR MARKERS FOR BACKGROUND SELECTION IN MUNGBEAN

Written by Ketwalee SIWAPITHAKPONG, Sukanya INTHAISONG, Witsarut CHUEAKHUNTHOD, Piyangkoon JAUKWON, Piyada Alisha TANTASAWAT
Pages 12 to 21

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REVIEW ON SECONDARY METABOLITES IN Salvia spp.

Written by Behnaz RAHMANIFAR, Oana LIVADARIU, Călina Petruța CORNEA
Pages 22 to 29

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PLANT SOMATIC EMBRYOGENESIS - A FOCUS ON THE ROLE OF CALCIUM IN EMBRYO INDUCTION AND DEVELOPMENT

Written by Ricardo MIR, Antonio CALABUIG-SERNA, Jose María SEGUÍ-SIMARRO
Pages 30 to 42

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AMINO ACID COMPOSITION AND ANTIOXIDANT ACTIVITY OF BIOLOGICALLY ACTIVE PREPARATIONS OBTAINED FROM WINE YEAST SEDIMENTS

Written by Oleg CHISELIȚA, Alina BEȘLIU, Natalia CHISELIȚA, Nadejda EFREMOVA, Elena TOFAN
Pages 43 to 48

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ANTIMICROBIAL ACTIVITY OF MICROORGANISMS ISOLATED FROM SILT OF THE "LA IZVOR" LAKE SYSTEM (CHISINAU MUNICIPALITY)

Written by Tamara SÎRBU, Svetlana BURȚEVA, Maxim BÎRSA, Nina BOGDAN-GOLUBI, Valerina SLANINA, Cristina MOLDOVAN, Olga ȚURCAN
Pages 49 to 60

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THE EFFECT OF Trichoderma AND DIATOMITE ON THE GROWTH AND DEVELOPMENT PARAMETERS OF SOME BELL PEPPER SPECIES

Written by Cristian-Alexandru TOADER, Floarea BURNICHI, Mona Elena POPA
Pages 61 to 66

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COMPARATIVE STUDY ON THE COMPOSITION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF FENNEL HYDROLATES

Written by Elena MIHAI, Alexandra GASPAR-PINTILIESCU, Teodora CIUCAN, Ana-Maria PRELIPCEAN, Justinian TOMESCU, Miruna NEAGU, Oana CRĂCIUNESCU
Pages 67 to 74

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THE INFLUENCE OF GROWTH-STIMULATING RHYSOBACTERIA (PGPB) ON THE PHYSIOLOGICAL PROCESSES AND PRODUCTIVITY POTENTIAL OF GRAPEVINES

Written by Tatiana DAVID, Leonid POPOV, Leonid VOLOSCIUC, Maria GAMURAR, Cristina MUNTEANU
Pages 75 to 80

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REVIEW ON DIFFERENT APPLICATIONS OF Lavandula spp. AS A SOURCE OF BIOACTIVE COMPOUNDS

Written by Ionuț Georgică MARCHIDAN, Mihaela GEICU-CRISTEA, Elisabeta Elena POPA, Gabriela BUTNARIU, Narcisa Elena BĂBEANU
Pages 81 to 96

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PREPARATION OF COMPOST FROM SEA BUCKTHORN BRANCHES BY USING A MULTIPURPOSE Trichoderma STRAIN

Written by Ionuț GIURESCU, Tatiana-Eugenia ȘESAN, Stere BADJU, Carmen LUPU, Florin OANCEA
Pages 97 to 104

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KERATIN EXTRACTION FROM CHICKEN FEATHERS IN AQUEOUS SOLUTIONS

Written by Victor Alexandru FARAON, Eliza Gabriela MIHĂILĂ, Naomi TRITEAN, Bogdan TRICĂ, Luiza CAPRĂ, Mihail-Bogdan ROMAN, Diana CONSTANTINESCU-ARUXANDEI, Florin OANCEA
Pages 105 to 112

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BLEACHING PRETREATMENT OF SULFITE PULP WITH COMMERCIAL MACERATION ENZYMES

Written by Victoria BÎNZARI, Ioana TUDOR, Denisa-Ioana GHEORGHE, Constantin ISVORANU, Diana CONSTANTINESCU-ARUXANDEI, Carmen LUPU, Mihai SÂRBU, Florin OANCEA
Pages 113 to 118

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EVALUATION METHODS OF MYCOTOXIGENIC CONTAMINANTS IN FEED RAW MATERIALS - A REVIEW

Written by Asawir Esamaldeen Ebrahim MOHAMED, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA
Pages 119 to 130

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TECHNOLOGIES FOR SOURDOUGH OBTAINING, FERMENTATION AND APPLICATIONS – A REVIEW

Written by Sabina-Andreea BOBEA, Nastasia BELC, Călina Petruța CORNEA
Pages 131 to 138

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BIOETHANOL PRODUCTION THROUGH Saccharomyces AND Non-Saccharomyces YEAST STRAINS ISOLATED FROM COCOA MUCILAGE JUICE FERMENTED

Written by Wahauwouele Hermann COULIBALY, Tano Marie-Ange Sakia MIAN, Fatoumata CAMARA, Grah Avit Maxwell BEUGRÉ, Camelia Filofteia DIGUȚĂ, Florentina MATEI
Pages 139 to 146

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MOISTURIZERS MADE WITH NATURAL INGREDIENTS

Written by Diana GROPOŞILĂ-CONSTANTINESCU, Gabriela MĂRGĂRIT, Gabriela POPA, Dana BARBA
Pages 147 to 152

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STUDIES REGARDING ANTIOXIDANT PROPERTIES OF ANTIMICROBIAL BIOPRODUCTS FORMULATED WITH NATURAL POLYMERS

Written by Mohammed Shaymaa Omar MOHAMMED, Nicoleta RADU, Mariana VOICESCU, Lucia Camelia PÎRVU, Narcisa BĂBEANU
Pages 153 to 158

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LIPOSOMES WITH PLANT EXTRACTS IN THE TREATMENT OF CARDIOVASCULAR DISEASES

Written by Ramona-Daniela PAVALOIU, Fawzia SHA’AT, Corina BUBUEANU, Paul BARBUTA, Ciprian FODOR, Mariana FODOR
Pages 159 to 167

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METHODS TO IMPROVE BIOAVAILABILITY OF NATURAL SENOLYTICS - A MINI REVIEW

Written by Dana MIU, Fawzia SHAAT, Ramona-Daniela PAVALOIU
Pages 168 to 173

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Ketwalee SIWAPITHAKPONG, Sukanya INTHAISONG, Witsarut CHUEAKHUNTHOD, Piyangkoon JAUKWON, Piyada Alisha TANTASAWAT

This study aimed to quantify the recurrent parent genome (RPG) recovery of the new mungbean introgression lines using SSR and EST-SSR markers, and selection of backcross progenies of the crosses between a resistant line (SUPER5) and two recurrent parents (KING and H3). A total of 160 SSR and EST-SSR markers covering 11 mungbean chromosomes were applied for parental polymorphism identification. Among these, 6 polymorphic markers were linked to domestication related traits located on linkage groups (LGs) 1, 3, 7 and 10. Twenty-seven (16.9%) and 23 (14.4%) SSR and EST-SSR markers were found to be distinct and clearly polymorphic between KING and SUPER5, and H3 and SUPER5, respectively. These polymorphic markers were utilized to analyse the RPG recovery in BC1F1 and BC2F1 progenies. The BC2F1 selected progenies with high RPG recovery ranging from 87.2-94.7% and 92.1-97.8% in KING and H3 populations, respectively, were further used to produce BC3F1 seeds. These results suggest that SSR and EST-SSR markers can be used in marker-assisted selection (MAS) for background selection to accelerate backcrossing.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Mohammed Shaymaa Omar MOHAMMED, Nicoleta RADU, Mariana VOICESCU, Lucia Camelia PÎRVU, Narcisa BĂBEANU

The antioxidant activity of antimicrobial bioproducts formulated with natural polymers most often depends on the activities of the active compounds from their composition. In the case of bioproducts with biopolymers, the antioxidant activities depend on the limonene source. In the present case, the antimicrobial activities of antimicrobial gels formulated with biopolymers were determined by the chemiluminescence method, using two sources of limonene, respectively the citrus essentials oils with 97% and respectively with 25% limonene. The results obtained from the chemiluminescence studies have shown that, if an essential oil (EO) with a high limonene content is used in the antimicrobial formulations, such as EO of Citrus sinensis (97% limonene), then the product will most often act as a prooxidant, due to the high limonene content. If the antimicrobial formulations are performed with less concentrated sources of limonene, such as the EO of bergamot (Citrus bergamia), then the resulting bioproducts, in addition to the maximized antimicrobial effect, will also act as antioxidants, the antioxidant activity obtained being 70%.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Sabina-Andreea BOBEA, Nastasia BELC, Călina Petruța CORNEA

Recent trends are towards long-life, nutritious, preservative-free products that improve immunity, digestion and well-being and are tailored to nutritional needs and lifestyle of the consumer. In this perception, sourdough bread is part of this product category. The sourdough has a key role in obtaining a product with sensory properties such as: color, flavor, taste, texture even bread volume. The microflora is dominated by lactic acid bacteria and yeast, very important elements when we talk about the fermentation of bread dough. The factors influencing dough quality are: dough yield, temperature, microflora, environmental acidity and substrate. Dough can be classified into 3 types, of which the most used in the bakery industry is Type III. The beneficial contribution that this type of fermentation brings is not only to increase the volume of the bread, the flavor and the nutritional value, but also to extend the shelf life by inhibiting spoilage bacteria.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Cristian-Alexandru TOADER, Floarea BURNICHI, Mona Elena POPA

Within the Vegetable Research and Development Station Buzău, aspects were determined and monitored regarding the effectiveness of products based on Trichoderma T85, administered at planting 3 granules/plant, for bell pepper culture and observations regarding the effectiveness of growth and development parameters for bell pepper, Cantemir (variety approved at V.R.D.S. Buzău). Trichoderma viride T85 is a fungal inoculant that was applied to variant V5 - three granules at planting and solid diatomite, which was incorporated into the soil during the vegetation period at V1, V2, V3, V4. The obtained results confirm the data presented in the specialized literature. Treatments with mixed bacterial preparations, with Trichoderma spp. and with solid diatomite can have a beneficial influence on the growth and development of vegetable species cultivated in organic or conventional systems, according to studies carried out over time, both in the country and abroad. This paper evaluates the effectiveness and benefits of using these bio-stimulants.

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