Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Fawzia SHAAT, Ramona-Daniela PAVALOIU, Ciprian FODOR, Emilian ROMAN, Mariana FODOR

Liposomes play a vital role in the advancement of regenerative cosmetics due to their capacity to encapsulate, protect, and effectively deliver active ingredients deep within the skin. These versatile carriers mimic biological membranes, enhancing the stability and bioavailability of sensitive compounds such as peptides, growth factors, vitamins, and plant-derived bioactives. Regenerative cosmetics leverage liposomal systems to promote skin repair, boost collagen synthesis, and combat signs of aging by targeting cellular pathways with precision. This review explores the state-of-the-art advancements in liposome technology for cosmetic applications, emphasizing their role in addressing challenges such as poor solubility, rapid degradation, and limited penetration of active agents. Additionally, we examine the economic viability, scalability, and regulatory considerations associated with integrating liposome-based products into the commercial cosmetic market. Despite their transformative potential, challenges remain in ensuring formulation stability, achieving cost-effective production, and verifying long-term safety. By providing a comprehensive overview, this review underscores the potential of liposome technology to revolutionize regenerative cosmetics, paving the way for next-generation skincare innovations that restore, rejuvenate, and protect the skin.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Behnaz RAHMANIFAR, Oana-Alina BOIU-SICUIA, Oana LIVADARIU, Niculina IONESCU, Călina Petruța CORNEA

Sage (Salvia sp.) is a key medicinal plant from the Lamiaceae family. Recently, the mass production of hairy roots has become important for producing plant secondary metabolites, offering fast growth and stable bioactive compound production, making them ideal for transgenic research. In this study, the optimization of hairy root induction conditions by Agrobacterium rhizogenes in Salvia officinalis, has been evaluated. For this purpose, the effect of 3 different times of bacterial co-culture (24, 48, 72 h) with explants (whole seedling, leaf, stem and petiole) was investigated. The 48h co-culturing interval was considered the most suitable time. The highest rate of success in hairy root production (88.4 %) belonged to the leaf explants. In order to evaluate the stability and quantity of hairy roots, liquid 0.5X MS medium with 3 treatments of IBA (0, 0.5, 1 mg/L) were used. The highest percentage of growth was observed in liquid 0.5X MS and at the hormone level of 1 mg/L. Transgenic hairy roots produced were confirmed by PCR.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Magdalina URSU, Nicoleta RADU, Silvana Mihaela DÄNÄILÄ‚-GUIDEA, Narcisa Elena BÄBEANU

The study aimed to evaluate the antimicrobial activity of certain bioproducts obtained from turmeric and ginger rhizomes, respectively, four essential oils (EO) purchased from NJoy and Oleya and four bioproducts obtained through chloroform and ethanol extraction. The results showed that the EO of ginger (NJoy) exhibits moderate antimicrobial activity against E. coli and significant antimicrobial activity on S. aureus, C. albicans, and C. parapsilosis; the EO of turmeric (Oleya) exhibit moderate antimicrobial activity for E. coli and C. albicans. The bioproduct, derived from the extract of turmeric in chloroform, has a significant antimicrobial effect on C. albicans and C. parapsilopsis. The alcoholic extracts of turmeric and ginger exhibit local antimicrobial effects on E. coli and no antimicrobial activity for C. albicans, C. parapsilopsis, or S. aureus. In conclusion, the preliminary tests achieved in vitro indicate significant differences between the tested bioproducts, some of these showing high potential for specific antimicrobial applications.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Ervin COZMA, David QUINTÍN MARTÍNEZ, Presentacíon GARCÍA GÓMEZ, Jose FERNANDEZ CALATAYUD, Florentina ISRAEL-ROMING

Circular economy is a sustainable approach that can help reduce waste, by valorising by-products and other materials for obtaining new products or for obtaining value added compounds. The aim of this study is to present a potential valorisation of various plant by-products through the brewing of Kombucha teas. This approach not only contributes to reducing food waste but also encourages the creation of more sustainable food systems by promoting resource efficiency. The plant-based Kombuchas were prepared separately using grape, lemon, broccoli, green tea and apple by-products. After the required aerobic fermentation process, the obtained samples showed regular pH values (3.3-3.5), Brix values (5.6-10.9), ethanol levels (<3 g/100 mL) and acidity levels (< 0.8% acetic acid). All of the samples, which were sterilised at 75 and 98°C respectively, showed no signs of any bacteria (Enterobacteriaceae and Listeria monocytogenes colonies) or fungi (yeasts and molds colonies). The results showed that discarded plant by-products have the potential to be used to obtain refreshing and natural Kombucha drinks.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Ivona Maria ZĂBAVĂ, Tudor Andrei KACENCO, Mădălina DEGENARO, Nicolae DOJANĂ

The biotechnology of producing egg-laying hen hybrids requires a high genetic selection pressure upon the origin inbred strains. The selection may affect the response of the reproductive system to the pituitary-ovarian axis control. In this work, we investigated the changes of ovarian and oviductal histomorphology response to the control exerted by the pituitary-ovarian axis in Lohmann and Leghorn hybrids, versus Sussex hens that has not been subjected to any selection pressure. Determinations were performed on 32-week-old hens, a key-age during the laying cycle. The results revealed differences between the hybrid hens and Sussex hens regarding the blood levels of gonadotropin follicle-stimulating hormone, luteinizing hormones (P<0.01), estrogen (P<0.05), triiodothyronine and tetraiodothyronine, and higher values of egg-laying frequency in hybrids versus Sussex. The number of different types of ovarian follicles (white follicles, small yellow and large yellow follicles), the structure of the follicle perivitelline membrane, and structure of the yolk was also modified in hybrids versus Sussex. Differences in epithelium histology of the infundibulum, magnum, isthmus and shell gland as well as histochemical particularities of these structures were also identified.

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DEVELOPMENT OF A BLUNT-END CLONING STRATEGY TO CONFIRM THE Rvi2 GENE IN APPLE VARIETIES CULTIVATED IN ROMANIA

Written by Adina Floricica IANCU, Mădălina MILITARU, Sina COSMULESCU
Pages 7 to 18

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INFLUENCE OF NATURAL AND SYNTHETIC SWEETENERS ON FLAVOPROTEINS FOUND IN BLACK TEA

Written by Constantin BUTOI, Alexandra Ioana IONESCU, Viorel SCHIOPU, Georgeta POPESCU
Pages 19 to 24

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OPTIMIZATION OF Agrobacterium rhizogenes - MEDIATED HAIRY ROOT INDUCTION IN Salvia officinalis

Written by Behnaz RAHMANIFAR, Oana-Alina BOIU-SICUIA, Oana LIVADARIU, Niculina IONESCU, Călina Petruța CORNEA
Pages 25 to 32

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ECOLOGICAL CONTROL OF POWDERY MILDEW OF CUCUMBER BY POTASSIUM PHOSPHATE AND NEEM OIL AND THEIR EFFECTS ON CONTENTS OF CHLOROPHYLLS IN LEAVES

Written by Vladimir ROTARU, Vladimir TODIRAȘ
Pages 33 to 38

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SUSTAINABLE AGRICULTURAL SOLUTIONS APPLIED TO BUCKWHEAT COVER CROP IN 2024

Written by Mirela DRĂGAN, Andrei PETRE, Silvana Mihaela DĂNĂILĂ-GUIDEA, Ștefana JURCOANE
Pages 39 to 44

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RESPONSE OF THE REPRODUCTIVE SYSTEM MORPHOLOGY TO CHANGES IN THE PITUITARY-OVARIAN AXIS CONTROL IN DIFFERENTLY SELECTED HEN STRAINS DURING EGG-LAYING CYCLE

Written by Ivona Maria ZĂBAVĂ, Tudor Andrei KACENCO, Mădălina DEGENARO, Nicolae DOJANĂ
Pages 45 to 51

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IMPORTANCE OF ZINC COMPOUNDS AND ZINC NANOPARTICLES IN DEVELOPMENT OF PLANTS

Written by Evelina GHERGHINA, Gabriela LUȚĂ, Daniela BĂLAN
Pages 52 to 58

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ASSESSING THE PHYLOGENY AND GENETIC VARIABILITY OF Orobanche cumana Wallr. POPULATIONS USING ISSR MARKERS

Written by Maria DUCA, Ina BIVOL
Pages 59 to 67

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EXPLORING THE BACTERIOCINOGENIC PROPERTIES OF Lactococcus lactis R152 ISOLATED FROM A TRADITIONALLY MADE CHEESE

Written by Ecaterina-Teodora CHIREA, Emanuela-Catalina IONETIC, Iulia-Roxana ANGELESCU, Silvia-Simona GROSU-TUDOR, Cristina PURCAREA, Medana ZAMFIR
Pages 68 to 74

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CHARACTERIZATION OF FUNCTIONAL GLUTEN-FREE BREADSTICKS WITH CUMIN IMPROVED BY ADDITION OF CRUCIFEROUS EXTRACTS

Written by Elena-Claudia STOICAN (GRADEA), Ionuț MORARU, Angela MORARU, Mircea-Cosmin PRISTAVU, Florentina ISRAEL-ROMING
Pages 75 to 83

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VARIATION OF FLAVOPROTEIN CONCENTRATION AFTER SWEETENING IN A GREEN-TEA BASED FOOD SUPPLEMENT

Written by Alexandra-Ioana IONESCU, Constantin BUTOI, Viorel SCHIOPU, Georgeta POPESCU
Pages 84 to 89

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RESEARCH ON THE VALORISATION OF PLANT-BASED BY-PRODUCTS TO PRODUCE FOOD LINKED TO THE CIRCULAR ECONOMY CONCEPT

Written by Ervin COZMA, David QUINTÍN MARTÍNEZ, Presentacíon GARCÍA GÓMEZ, Jose FERNANDEZ CALATAYUD, Florentina ISRAEL-ROMING
Pages 90 to 97

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GLOBAL TRENDS AND RESEARCH EVOLUTION IN FLOUR AND BAKERY PRODUCT FORTIFICATION: A BIBLIOMETRIC ANALYSIS

Written by Monica ȚUȚUIANU, Lavinia-Mariana BERCA, Dănuț CIMPONERIU, Florentina ISRAEL-ROMING
Pages 98 to 103

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THE LATEST DEVELOPMENT OF RADIO-FREQUENCY APPLICATIONS IN THE FOOD INDUSTRY: ADVANTAGES AND DRAWBACKS

Written by Amalia Carmen MITELUȚ, Georgiana APOSTOL, Elisabeta Elena POPA, Mihaela GEICU-CRISTEA, Mihaela DRĂGHICI, Paul-Alexandru POPESCU, Mona Elena POPA
Pages 104 to 113

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A CROSS-COUNTRY CONSUMERS’ STUDY ON THE ACCEPTANCE OF GRISSINI ENRICHED WITH WHEY

Written by Denisa Eglantina DUȚĂ, Gabriela Daniela CRIVEANU-STAMATIE, Cristian FLOREA, Mihaela ANGHEL (MULȚESCU), Adriana Laura MIHAI, Claudia ZOANI, Nastasia BELC, Michael RYCHLIK
Pages 114 to 120

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LIPOSOMES IN REGENERATIVE COSMETICS: REVOLUTIONIZING SKIN CARE THROUGH ADVANCED NANOTECHNOLOGY

Written by Fawzia SHAAT, Ramona-Daniela PAVALOIU, Ciprian FODOR, Emilian ROMAN, Mariana FODOR
Pages 121 to 130

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ANTIRHEUMATIC CREAM BASED ON NATURAL INGREDIENTS

Written by Diana GROPOŞILĂ-CONSTANTINESCU, Ioan-Nicolae RANGA, Radu-Cristian TOMA, Gabriela-Lucica MĂRGĂRIT
Pages 131 to 136

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THE ANTIOXIDANT POTENTIAL OF SOME Mespilus germanica L. EXTRACTS

Written by Elena BIRZA, Nicoleta RUSU, Cătălina VOAIDEȘ, Narcisa Elena BĂBEANU
Pages 137 to 147

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PRELIMINARY STUDIES REGARDING THE ANTIMICROBIAL PROPERTIES OF SOME BIOPRODUCTS DERIVED FROM Zingiber officinale AND Curcuma longa

Written by Magdalina URSU, Nicoleta RADU, Silvana Mihaela DÄNÄILÄ‚-GUIDEA, Narcisa Elena BÄBEANU
Pages 148 to 155

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Mirela DRĂGAN, Andrei PETRE, Silvana Mihaela DĂNĂILĂ-GUIDEA, Ștefana JURCOANE

In the context of the climate challenges of 2025, characterized by prolonged drought, buckwheat stands out as a sustainable solution for agriculture in Stupina Cornetu in Ilfov County, Romania. This plant, used as a cover crop, offers multiple benefits, including improving soil quality, reducing water loss and supporting biodiversity, while also being a valuable source of pollen for bees. Buckwheat sowing was carried out in June, in drought conditions, by using seeds of an adapted variety (Fagopyrum esculentum L. ʻZitaʼ), characterized by a short vegetation period and high tolerance to water deficit. The cultivation scheme included the use of technological links of minimal soil preparation in order to conserve moisture. The results obtained contributed to the creation of a sustainable agricultural ecosystem through the cultivation of buckwheat plants. This culture can thus become a key component in adapting to climate change and supporting a sustainable agriculture in Romania.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Elena BIRZA, Nicoleta RUSU, Cătălina VOAIDEȘ, Narcisa Elena BĂBEANU

This study aimed to evaluate the antioxidant activity and mineral content of medlar fruit (Mespilus germanica L.) extracts, using different solvents to identify the most effective extraction method. Extracts were prepared using acetone, ethanol, water, and chloroform. Polyphenol content was analyzed using HPLC, minerals were determined using inductively coupled plasma mass spectrometry (ICP-MS), and antioxidant activity was assessed using chemiluminescence assays to determine IC50 values. The acetonic extract (MP1) had the highest polyphenol content and strong antioxidant activity (AA% of 64%). The aqueous extract (MP2) was richest in minerals. The ethanolic extract (MP3) balanced polyphenol content and antioxidant activity (AA% of 51%). The chloroform extract (MP4) showed a pro-oxidant effect (AA% of -151%). Acetone is the best solvent for extracting polyphenols, while water is ideal for mineral extraction. The ethanolic extract provides a balanced option. Chloroform extracts should be used with caution due to potential pro-oxidant effects. These findings help optimize extraction methods for health benefits, with potential applications in food and pharmaceuticals.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Amalia Carmen MITELUȚ, Georgiana APOSTOL, Elisabeta Elena POPA, Mihaela GEICU-CRISTEA, Mihaela DRĂGHICI, Paul-Alexandru POPESCU, Mona Elena POPA

The quest for innovative processing solutions that meet both food safety and quality standards and sustainability goals (in terms of processing, preservation, and consumption) has led to a growing interest in technologies like radiofrequency (RF) technology. Although the mechanisms behind this technology have been thoroughly explained and are well-documented in the literature, and its advantages for industrial applications are recognized in several studies, the widespread adoption of RF technology for various agri-food products thermic treatment, remains limited due to unsolved issues. One of the main challenges in applying RF technology on a large scale is the need to adapt equipment and technology according to the specific characteristics of the product (e.g. raw material, finished product, liquid, semi-liquid, solid, packaged, unpackaged) and the intended purpose (e.g., processing, preservation, sanitization). This paper aims to review recent findings in this area, highlighting both the benefits and challenges of RF technology application for different food products thermal processing. Addressing these challenges requires continued scientific research in various directions within this field.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Alexandra-Ioana IONESCU, Constantin BUTOI, Viorel SCHIOPU, Georgeta POPESCU

Flavoproteins are proteins that contain a nucleic acid derivative of riboflavin. Flavoproteins removes the free radicals that contributes to oxidative stress and have an important role in DNA repair. The raw material used, is free from plant growth hormones and pesticides, helping to maintain high levels of nutrients in the finished product. The objective of this paper work is to identify the variant with the highest concentration of flavoproteins following the green tea sweetening process, in order to produce the most effective product for an innovative food supplement. The results of this phase of research have identified the variant with the highest concentration of flavoproteins following the green tea sweetening process. The best variant obtained at this stage of research is characterized by sensory improvements and minimal changes in chemical composition compared to the unsweetened variant.

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