PHENOLIC CONTENT AND POTENTIAL INHIBITORY ACTIVITY OF ROMANIAN BEE POLLEN ON DIFFERENT PLANT PATHOGENIC STRAINS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Roxana SPULBER, Mariana-Graţiela VLADU, Ovidiu POPA, Narcisa BĂBEANU

Bee pollen is a fine powder gathered from different plant species, enriched and transformed by bees into a complex bee product. Most bioactive properties of bee pollen, including antimicrobial activity, have been attributed to phenolic compounds. The aim of this study was determination of botanical origin, total phenolic content, flavonoid content and antimicrobial properties of three different pollen types from Romania against some plant microbial pathogens. Due to the fact that bioactive compounds of bee pollen depends strongly on the plant source, we tested extracts of fresh monofloral and polyfloral bee pollen. The ethanol 70% was used for pollen extraction. In this study, three microbial agents were tested: two bacterial strains represented by Erwinia carotovora (subsp. carotovora) ICCF 138, Xanthomonas campestris ICCF 274 and one fungal strain represented by Aspergillus niger ICCF 92. Furthermore, total phenolic and flavonoid contents were carried out using Folin-Ciocalteau procedure and aluminum chloride spectrophotometric method, respectively. Qualitative screening of pollen extracts antimicrobial activity was tested by disc diffusion method. The palynological investigations allowed us to identify the botanical origin of bee pollen loads, represented by species of eight different genera. Pollen sample extracts revealed high values for phenolic and flavonoid contents and also demonstrated that possess antimicrobial activities. We tried to established if the total phenolic or flavonoid content were related to the phytopathogenic antimicrobial activity. Our results indicated that bee pollen could be considered a promising natural source of plants protection.

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SPULBER R., VLADU M.G., POPA O., BĂBEANU N. 2017, PHENOLIC CONTENT AND POTENTIAL INHIBITORY ACTIVITY OF ROMANIAN BEE POLLEN ON DIFFERENT PLANT PATHOGENIC STRAINS. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 104-108.

PHYSICAL AND CHEMICAL CHARACTERISTICS OF CROSSED OLIVES AND THEIR CONVENIENCE TO GREEN TABLE OLIVE FERMENTATION BY USING Lactobacillus plantarum AS A STARTER CULTURE

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Yasin OZDEMIR, Sefik KURULTAY

Genetic variation was reported as an important factor effects quality of table olive. So that researchers aimed to develop new cultivar which had high table olive characteristics than that’s of standard cultivar. This research was aimed to determine characters of raw and processed fruits of 4 crossed olive genotypes which had been reported by previous studies as promising cultivar for registration according to agronomic characteristics. Fruits of Manzanilla cultivar which is the most important green table olive cultivar in Spain were used for comparison. Number of olives per kilogram, flesh to seed ratio, water, oil, total and reducing sugar, and phenolic compounds were analyzed. Sensory and salt analyses also were applied to processed olives. For green table olive production; olives were debittered by 2% NaOH and then put in brine which contained 5% salt at pH4,5. At 4th day of keeping the olives in brine, 107CFU/mL Lactobacillus plantarum were inoculated to the brine for fermentation until pH fall to 3,8.All the olives of genotypes had enough reduced sugar content (>2 %) for fermentative microorganisms and higher olive weight than Manzanilla but only olive of BK013 had higher flesh to seed ratio than Manzanilla. After processing hydroxytyrosol losses were determined in the range of 30,25-88,88 % and processed olives of MT038 had higher hydroxytyrosol content this is precious for nutrition physiology of consumer. Olives of BK013 and GK131had bettertable olive and sensory characteristics so that they have potential for registration as new table olive cultivar.

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OZDEMIR Y., KURULTAY S. 2016, PHYSICAL AND CHEMICAL CHARACTERISTICS OF CROSSED OLIVES AND THEIR CONVENIENCE TO GREEN TABLE OLIVE FERMENTATION BY USING Lactobacillus plantarum AS A STARTER CULTURE. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 154-161.

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PHYSICAL CHEMICAL STUDIES REGARDING CIDER STABILITY STORED UNDER AMBIENT CONDITIONS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Iuliana BELEUZU BODA, Marius Cristian BODA

Multiple cider samples obtained from United Kingdom market were analyzed for their parameters before opening and after 24/48 hour storage on ambient conditions. We aimed to study the basic parameters such as color, turbidity and sulfites contained in different type of ciders. The first step to achieve our general aim was to select from the diversity of cider market and to assess the parameters used to conduct this study. The physical and chemical studies allow us to evaluate not only the diversity of the samples but also the how they behave in different ambient conditions. This information is essential for the selection of cider consumption and for better understanding of the selected parameters for further beverage studies, especially apple and pear made ones. Deviant variation from standard was found in more than half from twenty five samples analyzed. The results of previous studies showed that the defective storage conditions of cider might affect not just the bacteria contaminations, but that include also defective physical and chemical composition. The experiments exposed that weaknesses of the opened product that might become a quality, or more than that, a safety issue through its flawed parameters when these common conditions exist.

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BELEUZU BODA I., BODA M.C. 2016, PHYSICAL CHEMICAL STUDIES REGARDING CIDER STABILITY STORED UNDER AMBIENT CONDITIONS. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 129-136.

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PHYSICO-CHEMICAL AND MICROBIOLOGICAL CHARACTERIZATION OF WHEAT FLOURS ON ROMANIAN MARKET IN RELATION TO THE SHELF LIFE

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Radiana TAMBA-BEREHOIU, Ciprian–Nicolae POPA, Vasilica SIMION, Rodica CULEA

This paper aims to assess the quality and microbiological parameters of a range of wheat flours for domestic consumption, purchased on the Romanian market. In this regard there were purchased 20 samples of flour from 13 manufacturers. Quality parameters analyzed were: Moisture (%), Protein content (%), Ash content (%), Water absorbtion (%), Total combined Yeasts and Molds count (CFU/g) and the number of days until the deadline of the shelf life. The analyzed flours were characterized by the following variation ranges of the parameters: Moisture (%) 10.9 - 14.4, Protein content (%) 10.0 - 15.7, Ash content (%) 0.40 - 1.59, Water absorbtion (%) 57.0 - 61.9, Yeast and molds (CFU/g): 10-410, and number of days until the deadline of the shelf life: 9-326. The results showed that there are no significant correlations between analyzed parameters and the content of yeasts and molds. This suggests that the dynamics of yeasts and molds population in packed flours is dependent primarily on the processing conditions and less on the factors that act during the lifetime of the product on the shelf.

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TAMBA-BEREHOIU R., POPA C.N., SIMION ., CULEA R. 2016, PHYSICO-CHEMICAL AND MICROBIOLOGICAL CHARACTERIZATION OF WHEAT FLOURS ON ROMANIAN MARKET IN RELATION TO THE SHELF LIFE. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 218-221.

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PHYTOHORMONE–LIKE PRODUCING BACILLUS INCREASE TOMATO SEEDLINGS QUALITY

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Oana-Alina SICUIA, Sorina DINU, Florica CONSTANTINESCU

Plant growth promoting activity is one of the attributes when searching for beneficial strains of bacteria. Our study aimed to present the potential of Bacillus sp. 83.2s and B. subtilis Bce2 to increase plant growth and vigour. The study is based on phytohormone - like evaluation in the selected Bacillus strains using classic biochemical analysis. Likewise, the plant beneficial effect was also evaluated by growth promotion studies on tomato seedlings, were biometric parameters and chlorophyll content index (CCI) were analyzed. The data have been processed into the following indicators: indole-3-acetic acid in bacterial cultures, and emergence, seedling height and vigour, shoot and root dry weight and CCI in tomatoes seedlings. Both bacterial strains produce high amounts of IAA phytohormones, from 10.4 - 13.8 μg/ml, in normal growth medium, up to 16.3 - 16.6 μg/ml, when 5 mM of tryptophan is added in the medium as auxin precursor. Moreover, B. subtilis Bce2 seed treatment, increased emergence index and seedlings vigour compared to the untreated control and exceeded the commercial growth regulator (Vimpel 77%) in terms of emergence percent, seedlings dry weight, and chlorophyll content index.

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SICUIA O.A., DINU S., CONSTANTINESCU F. 2016, PHYTOHORMONE–LIKE PRODUCING BACILLUS INCREASE TOMATO SEEDLINGS QUALITY. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 83-88.

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POLYAROMATIC HYDROCARBONS UTILIZATION BY A Pseudomonas STRAIN

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Mihaela Marilena STANCU

Polyaromatic hydrocarbons (PAHs) are major environmental pollutants, which are well known for their toxic effects on the organisms. However, different Pseudomonas strains are able to use these toxic compounds as growth substrate. Pseudomonas aeruginosa strain IBBPo16 was able to grow on nutrient-rich and minimal media in the presence of several toxic PAHs, such as naphthalene, methylnaphthalene, phenanthrene, fluorene. P. aeruginosa IBBPo16 cells exhibited a higher growth when they were inoculated on nutrient-rich medium in the presence of PAHs, as compared with growth on minimal medium. Naphthalene was less toxic for P. aeruginosa IBBPo16 cells, compared with methylnaphthalene, phenanthrene, and fluorene. P. aeruginosa IBBPo16 cells grown in the presence of PAHs produced specific extracellular secondary metabolites (i.e., surfactants, pigments). P. aeruginosa IBBPo16 cells grown in the presence of naphthalene possess ndoM (naphthalene dioxygenase) and rhlAB (rhamnosyltransferase 1) genes, whereas C23DO (catechol 2,3-dioxygenase) and pahDO (PAH dioxygenase) genes were not detected in this Gram-negative bacterium.

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STANCU M.M. 2017, POLYAROMATIC HYDROCARBONS UTILIZATION BY A Pseudomonas STRAIN. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 200-205.

POTENTIAL USE OF KOMBUCHA CRUDE EXTRACT IN POSTHARVEST GRAPE MOULDS CONTROL

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Bogdan MATEI, Florentina MATEI, Camelia DIGUȚĂ, Ovidiu POPA

Postharvest diseases of fruits and grapes are caused by fungi and bacteria and the losses in this step increase several folds than in the field. In the case of the grapes, most damages are due to the presence of filamentous fungi belonging to species like Botrytis sp., Penicillium sp. or Aspergillus sp. Several non-chemical treatments have been proposed for fungal decay control, including acetic acid. Our experiments have targeted the potential inhibitory effect of different tea sourced Kombucha crude extracts on the most common moulds of the grapes in pre and postharvest steps. Kombucha is known mainly as a consortium (SCOBY - symbiotic acetic/lactic bacteria and yeast). Because of the presence of acetic bacteria the final content in acetic acid of Kombucha suspensions vary between 8.5 and 17 g/l. Kombucha tea suspension has been prepared starting from three different source of tea plants, respectively green tea, green tea with Melissa officinalis L. and oolong tea. The most significant inhibition has been registered in the case of Botrytis cinerea (38 -55%), less significant on Penicillium expansum (4-8%) and not significant on Aspergillus flavus and Aspergillus carbonarius. It is proposed further to investigate the inhibition of Kombucha extracts, in vivo, on artificially infected grape berries with Botrytis cinerea and to validate the in vitro results.

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MATEI B., MATEI F., DIGUȚĂ C., POPA O. 2017, POTENTIAL USE OF KOMBUCHA CRUDE EXTRACT IN POSTHARVEST GRAPE MOULDS CONTROL. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 77-80.

PRELIMINARY RESEARCH ON ENERGETIC CAPITALIZATION OF LIGNOCELLULOSIC MATERIALS IN FORM OF BIOETHANOL

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Diana GROPOȘILĂ-CONSTANTINESCU, Gabriela MARGĂRIT, Radu-Cristian TOMA, Dana BARBA, Luminița VIȘAN, Marius HANGAN

The huge amount of biomass composed of plant residues considered "waste" is a potential source of useful products. Works of this research were based on the study of the bioconversion potential in bioethanol of different types of wastes of lignocellulosic nature, obtained from the harvesting and processing of cereals (straw, cobs). Efficient capitalization of plant biomass is only possible when effective methods of delignification and decrystallization of the lignocellulosiccomplex are applied. Our research focused on the following general objectives: selection and characterization of lignocellulosic waste that can produce bioethanol, selection and adaptation of highly-productive microorganisms that ensures high conversion yields of bioethanol from the substrates obtained from lignocellulosic waste, experimentation of the technology at micropilot level. The raw materials used, wheat straw, barley straw and corn cobs, have been subjected to thermal pretreatment (autoclaving at 121°C for 30 minutes), enzymatic pretreatment (laccase), chemical pretreatment (NOH) and enzymatic hydroyisis (MethaPlus), in order to make available the polysaccharide substrates for the subsequent enzymatic hydrolysis. In case of alkaline pretreatment applied to all three types of lignocellulosic materials, best results were obtained when a solution of NaOH 4% was used. Anaerobic digestion of lignocellulosic materials led to ethanol concentrations of 4,9%, in case of corn cobs, 3,2% for wheat straw and 3.9, for barley straw.

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GROPOȘILĂ-CONSTANTINESCU D., MARGĂRIT G., TOMA R.C., BARBA D., VIȘAN L., HANGAN M. 2017, PRELIMINARY RESEARCH ON ENERGETIC CAPITALIZATION OF LIGNOCELLULOSIC MATERIALS IN FORM OF BIOETHANOL. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 190-193.

PRELIMINARY RESULTS OF THE WINTER PEAS BREEDING PROGRAM

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Ancuţa CRÎNGAŞU (BĂRBIERU)

The development of the winter pea crop represent a major challenge to expand plant protein production in temperate areas. Breeding winter cultivars requires the combination of freezing tolerance as well as high seed productivity and quality. Winter peas have some advantages over spring peas like: better establishment and more efficient use of humidity during the winter season, which makes it less vulnerable to drought over the spring, frequently in Romania in the last years; winter peas can be sown in mixture with some cereal (barley, triticale, grasses) for obtaining high nutritive green forage; earlier harvest; has a longer vegetation period and get higher productivity and more stable yield than spring peas type. In this paper we present data obtained from the first F3 lines of winter peas obtained in the NARDI-Fundulea program with the germplasm of winter peas from USA and Austria. A number of 176 lines, selected from winter/winter and winter/spring crosses pea genotypes, have been tested in preliminary trials in 2015. Data for yield showed a large variation, but some lines over yield significantly the winter control (Specter, Checo and Windham). The conclusion of this preliminary study is that will be possible to realize the genetic progress in breeding in winter peas, to select the new varieties with good enough winter hardiness and being with high yield, different earliness or plant height. Of course, the breeding program just started, from a short time, and it is needed to improve the genetic bases of the germplasm use for all traits, but mainly for winter hardiness.

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CRÎNGAŞU (BĂRBIERU) A. 2016, PRELIMINARY RESULTS OF THE WINTER PEAS BREEDING PROGRAM. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 22-26.

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PRELIMINARY RESULTS REGARDING THE TESTING OF TREATMENTS WITH LIGHT-EMITTING DIODE (LED) ON THE SEED GERMINATION OF ARTEMISIA DRACUNCULUS L.

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Irina-Maria ENACHE, Oana LIVADARIU

Regardless of the food diet (raw, vegan, vegetarian, carnivore), consumption of sprouted grains of different plants, especially vegetables, is encouraged, due to their therapeutic, regenerative and alimentary benefits. Seeds and sprouts have in their composition 20 - 30 times higher amounts of enzymes, minerals and nutrients than mature plants. Artemisia dracunculus L. (tarragon) is a medicinal and aromatic perennial herb which belongs to the Asteraceae family. It was chosen for the experiment due to its benefits, the essential oil of tarragon displaying antibacterial, antioxidant, antihyperglicemic activity. The plant is also known for enhancing digestion and having a pleasant spicy aroma. Studies show that light-emitting diode (LED), light exposure improves the quality of the growth, metabolism and accumulation of bioactive substances. As a result, the effect of different LED light colors on Artemisia dracunculus L. (tarragon), seed germination was studied. The Artemisia dracunculus L. (tarragon), seed were exposed to white, red, blue or green LED light, over a photoperiod of 16 hours, for seven days. The red LED exposure determined a higher degree of germination and longer hypocotil height. The blue LED exposure determined a better development of cotyledons. The experimental results obtained contribute with useful information in order to establish a method of easily growing fresh sprouts of Artemisia dracunculus L. (tarragon), for therapeutic and culinary use, under exposure to a low-carbon, power saving and inexpensive lightning means.

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ENACHE I.M., LIVADARIU O. 2016, PRELIMINARY RESULTS REGARDING THE TESTING OF TREATMENTS WITH LIGHT-EMITTING DIODE (LED) ON THE SEED GERMINATION OF ARTEMISIA DRACUNCULUS L.. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 51-56.

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