INFLUENCE OF CULTURE CONDITIONS ON GROWTH OF KERATINOPHILIC FUNGAL STRAINS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Mariana CĂLIN, Iuliana RĂUT, Diana CONSTANTINESCU-ARUXANDEI, Mihaela BADEA DONI, Melania-Liliana ARSENE, Nicoleta-Olguţa CORNELI, Gelu VASILESCU, Luiza JECU, Mariana Ştefania BUCUR, Veronica LAZĂR

The aim of this study was to investigate the influence of culture conditions on growth of keratinophilic fungal strain of Chrysosporium sp. in the presence or absence of keratin substrate. The effect of pH, temperature, carbon and nitrogen sources on fungal growth and sporulation was evaluated. The pH values ranged from 4 to 9.5 and the incubation temperature ranged from 20°C to 35°C. Glucose, fructose, maltose, sucrose, starch and cellulose were used as carbon sources. As nitrogen source, yeast extract, ammonium salts, urea and vitamin B12 were tested. All tests were also performed with basal mineral culture medium supplemented with keratin powder from chicken feathers. The feathers were cleaned with ethylic alcohol, washed with distilled water, dried at 60°C and finally grounded several times with a Retsch ball mill until a fine powder was obtained. The influence of the culture conditions on growth was assessed by measuring the diameter of the colonies grown on the solid medium after 5 and 10 days of incubation. The colony sporulation degree was appreciated macroscopically and microscopically. The presence of keratin in the culture media stimulated distinctly the fungal growth as compared to the culture media without keratin. Alkaline pH and temperatures between 27 and 30°C are optimal for its growth. Certain C and N sources can stimulate the fungal growth, but this seems to be influenced by the incubation time.

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CĂLIN M., RĂUT I., CONSTANTINESCU-ARUXANDEI D., BADEA DONI M., ARSENE M.L., CORNELI N.O., VASILESCU G., JECU L., BUCUR M.S., LAZĂR V. 2017, INFLUENCE OF CULTURE CONDITIONS ON GROWTH OF KERATINOPHILIC FUNGAL STRAINS. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 173-178.

INFLUENCE OF DIETHANOLAMINE SALT OF 4-NITROBENZOIC ACID IN CALLUS CULTURE AT MOMORDICA CHARANTIA L.

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Alina SIMINA, Manuela CRISAN, Sorin CIULCA, Dorica BOTAU

Medicinal plants and their products are an important solution to improve the treatment of people in the whole world. Momordica charantia L. is a well-known species for its biological activity (antioxidant and antimicrobial activity) and contains a complex of beneficial compounds such as: vitamins, minerals and antioxidants that can be used for treating a wide range of illnesses, especially diabetes. Plant tissue culture is an important and facile method for the somatic variability induction and tissue lines selection inorder to obtain valuable secondary metabolites. The use of the substances that control growth and synthesis capacity of tissues allow us to produce under aseptic conditions significant quantities of plant metabolites. The controlled conditions give to the tissue culture a suitable microenvironment for the successful growth and biosynthesis. Phytohormons and other substances with the same effect can determine in tissue culture the increase of biosynthetic capacity which can lead to obtaining and selection of proliferative tissue lines producing secondary metabolites. The present work aims to study the influence on M. charantia L. tissue culture of a new biological active compound, diethanolamine salt of 4-nitrobenzoic acid (4-NO2BA DEA), synthesized by the Institute of Chemistry Timisoara of Romanian Academy. In this research we used 6 hormonal balances in which we associated 4-NO2BA DEA with cytokinin BAP and also the new compound alone on the MS culture medium, for the selection of tissue lines with high growth capacity.We note that increasing the amount of 4-NO2BA DEA in the MS culture medium could have beneficial effects on tissue culture at M. charantia.

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SIMINA A., CRISAN M., CIULCA S., BOTAU D. 2016, INFLUENCE OF DIETHANOLAMINE SALT OF 4-NITROBENZOIC ACID IN CALLUS CULTURE AT MOMORDICA CHARANTIA L.. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 89-92.

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INFLUENCE OF DIFFERENT IN VITRO SIMULATORS FOR HYDRIC STRESS FOR GROWTH AND DEVELOPMENT OF POTATO

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Andreea TICAN, Mihaela CIOLOCA, Nicoleta CHIRU, Carmen BĂDĂRĂU

Necessity of finding genotypes adapted to drought has become urgent due to the effect of this type of stress on potato production. The most important phase, indispensable for improving drought tolerance is to identify genotypes tolerant and sensitive to drought. In this study, to induce in vitro water stress were used polyethylene glycol and sorbitol that was comparable with the basic medium MS, considered control. Determinations were performed 4 weeks after inoculation of mini cuttings belonging to five varieties of plantlets (Ruxandra, Sarmis, Gared, Marvis, Rustic) and the parameters analyzed were next: number of leaves, number of internodes, height of plantlets, root length, weight of fresh plantlet, weight of fresh root. Medium in which was added PEG with different concentrations significantly reduced the average weight of fresh plantlet and root compared with the control medium and medium with sorbitol and significantly reduced the mean number of internodes, the average height of the plantlet, the average root length. This osmotic agent (PEG) can be recommended for in vitro simulation of drought to identify tolerant genotypes to hydric stress.

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TICAN A., CIOLOCA M., CHIRU N., BĂDĂRĂU C. 2016, INFLUENCE OF DIFFERENT IN VITRO SIMULATORS FOR HYDRIC STRESS FOR GROWTH AND DEVELOPMENT OF POTATO. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 105-112.

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INFLUENCE OF TECHNOLOGICAL FACTORS ON ACRYLAMIDE LEVEL FROM BISCUITS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Mioara NEGOIȚĂ, Adriana Laura MIHAI, Enuța IORGA

The objective of this study was to investigate the influence of wheat flour extraction degree and baking parameters on acrylamide (AA) level in biscuits by using gas chromatography tandem mass spectrometry (GC-MS/MS). Color parameters were analyzed in an attempt to establish correlations with the investigated factors. Biscuits baked between 25 min ÷ 60 min at 200°C recorded values of AA level < LOD (4.63 μg/kg) to about 1.580 μg/kg. By baking biscuits at a temperature varying between 220°C ÷ 240°C for 20 min the AA level increased from 67.44 to 212.87 μg/kg. The highest AA level and the darkest color was obtained when using in the biscuits recipe the whole-wheat flour (F3) with an ash content of 2.37% d.m. (1580.33 μg/kg, ΔE*= 35.66), followed by white flour F1 with 0.53% d.m. (387.82 μg/kg, ΔE* = 61.79), respectively F2 with 0.44% d.m. (308.38 μg/kg, ΔE* = 67.47). From the results obtained it can be said that the AA level is strongly influenced by biscuits baking parameters (temperature and baking time) and by the ash content of wheat flour used in the manufacture recipe.

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NEGOIȚĂ M., MIHAI A.L., IORGA E. 2017, INFLUENCE OF TECHNOLOGICAL FACTORS ON ACRYLAMIDE LEVEL FROM BISCUITS. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 149-158.

INVESTIGATION OF SOME EXTRACTION METHODS FOR THE RECOVERY OF PEANUT PROTEINS FROM OILS AND FATS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Elena MIHAI (DRĂGHICI), Maria PELE

Food allergies have a considerable impact on modern society. There is no known cure. As a result, consumers can only avoid offending foods and use pharmacological agents. Some of the most severe allergic reactions occur when peanuts and peanut derivatives as peanut oils are consumed. The food industry will have to comply with requirements set forth by law for all packaged foods sold in the European Union. At times, it can be difficult to measure allergenic proteins in a wide variety of foods. Yet the food matrix can sequester allergens, inhibiting their detection, without significantly affecting allergenicity. The studies about allergenicity of edible oils and related to peanut oils are few and enough controversies. Some studies showed the presence of peanut allergens some not. It has to be emphasised that different studies used different methods for extraction, concentration and detection the peanut traces so the results had difficult been compared. In this context we investigated some extraction and concentration methods for the recovery of proteins from oils and fats derived from, or containing, peanut. The recovery of total protein and peanut allergens are very different for each method. Our result show how much the results depend on the method used to extract or/and concentrate the proteins from different matrices. The influence of solvent plays an important role in that process. Interactions with lipids of protein may alter the possibility to detect and quantify them by a hiding allergen/protein effect.

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MIHAI (DRĂGHICI) E., PELE M. 2016, INVESTIGATION OF SOME EXTRACTION METHODS FOR THE RECOVERY OF PEANUT PROTEINS FROM OILS AND FATS. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 204-209.

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ISOLATION OF FUNGAL MICROBIAL STRAINS FROM GIURGIU NORD TECHNOLOGICAL PARK WASTEWATER TREATMENT PLANT

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Ovidiu IORDACHE, Iuliana DUMITRESCU, Floarea PRICOP, Elena VĂRZARU, Cornelia MITRAN, Andreea CHIVU, Steliana RODINO

In the present study, several microbial strains were isolated in pure cultures from wastewater treatment plant of Giurgiu Nord Technological and Industrial Park, from five withdrawal points along the treatment process: point A, as the entry point of total water content after textile technological processes; point B, after colloidal particles removal stage with Al2(SO4)3 coagulant; point C, after mechanical filtration, chemical treatment and sedimentation process stages, point D, from the water obtained after applying treatment technological stages, mixed with sewage water, plus point E, from the soil located in the vicinity of the treatment plant, characterized by high microbial load. Four semisynthetic agarized nutritive media (PDA, MA, Czapek-Dox, Sabouroud), supplemented with 0.5% chloramphenicol for inhibition of certain bacterial species, were used for isolation of microbial load from the targeted samples. Highest microbial loads and variety were highlighted on plates isolated from soil (E samples), followed by A samples, B samples, and D samples, while C samples registered the lowest growth yield, possible due to inhibitory action of Al2- (SO4)3 coagulant. Morphological analysis of the obtained cultures revealed both filamentous fungi strains specific growth) and bacterial growth. Isolated strains will be used in further tests, as both inactivated and viable microbial biosorbents, for removal of specific wastewater pollutants from aqueous solutions.

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IORDACHE O., DUMITRESCU I., PRICOP F., VĂRZARU E., MITRAN C., CHIVU A., RODINO S. 2017, ISOLATION OF FUNGAL MICROBIAL STRAINS FROM GIURGIU NORD TECHNOLOGICAL PARK WASTEWATER TREATMENT PLANT. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 194-199.

LEATHER HYDOLYSATE EVALUATED AS BIOACTIVE POTATO FERTILIZER

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Mioara Ancuța DUMITRU, Gabriel CORBU, Ștefana JURCOANE

Leather industry discharges enormous amount of chrome containing leather solid wasted which creates a major disposal problem. Tanned leather solid waste is a complex of hard-to-degrade proteins and chromium. The biotechnological sector allows us to use the waste materials as bacterial substrate for enzyme production. The present work covers potential application in the potato bio-growth as fertilizer. The hydrolysate results from bacterial conversion of leather components. Bacteria was isolated from the composting of leather and incubated into a minimal media for 120 hours at 35°C. In the optimization process maximum proteinase production was 1.223 U/ml. The results obtained suggested that leather debris containing amino-acids and proteins andcan be applied as organic nitrogen soil input.

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DUMITRU M.A., CORBU G., JURCOANE S. 2016, LEATHER HYDOLYSATE EVALUATED AS BIOACTIVE POTATO FERTILIZER. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 40-43.

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LEVAN - A MINI REVIEW

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Caterina TOMULESCU, Roxana STOICA, Claudia SEVCENCO, Angela CĂŞĂRICĂ, Mişu MOSCOVICI, Adrian VAMANU

This review aimed to present a short summary of the biosynthesis, properties and industrial applications of levan, as a multiuse biopolymer. During the past years, a great number of bacterial polysaccharides have been discovered and nowadays, many studies about their molecular structure, biosynthesis and industrial development, or their functional properties establish correlations emphasizing their significant industrial value, especially as biomaterials. Levan and inulin are the main representative molecules, in the fructans group (as non-structural carbohydrates - fructose polymers). Levan is an extracellular polysaccharide (EPS), a biologically active polymer. It is a naturally occurring homopolymer of fructose, which can be found in plants and many microbial strains. Its main plant sources are: Agropyron cristatum, Dactylis glomerata, Poa secunda, Triticum aestivum, Cocksfoot and Pachysandra terminalis. As an EPS, levan is also produced, usually from sucrose-based substrates, by a variety of microorganisms: the most known microbial levan producers belong to the genera Zymomonas, Bacillus, Acetobacter, Aerobacter, Pseudomonas, Erwinia, Gluconobacter, Streptococcus and Corynebacterium. Many research works attribute levan a variety of potential applications in various fields, like: medical, chemical, pharmaceutical, cosmetics and food industries. General properties like film-forming ability, flexibility, renewability, biocompatibility, biodegradability and ecofriendliness, along with a number of remarkable physical, chemical and biomedical properties, made levan a superior biopolymer in many commercial sectors.

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TOMULESCU C., STOICA R., SEVCENCO C., CĂŞĂRICĂ A., MOSCOVICI M., VAMANU A. 2016, LEVAN - A MINI REVIEW. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 309-320.

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LIFE CYCLE ASSESSMENT (LCA) ON EUROPEAN SKIMMED MILK POWDER PROCESSING PRODUCTION PLANT

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Magdalini KROKIDA, Maria TAXIARCHOU, Antonis POLITIS, Antonis PEPPAS, Konstantina KYRIAKOPOULOU

The dairy industry consists one of the most energy intensive food industries, with milk powder production being the most energy consuming process. The aim of this work is to present the state of the art skimmed milk powder production processing chain in order to identify the processes with high environmental and energy impact. A life cycle assessment (LCA) has been performed to analyse the environmental footprint and energy balance derived from the skimmed milk powder (medium heat) production on the post-harvest chain. Therefore, a comparative gate to gate LCA was performed within the boundaries of the processing plant (i.e. standardization/separation, homogenization, pasteurization, evaporation, spray drying). In this study, two scenarios were evaluated on their environmental performance: a) the conventional production of skimmed milk powder (SMP) with the inclusion of Reverse Osmosis (Scenario 1) and b) the production of SMP exclusion of Reverse Osmosis (Scenario 2). The standard framework of LCA was followed according to the ISO 14044, which is also in line with the International Reference Life Cycle Data System (ILCD) Handbook. LCA study was performed on Gabi 6 software with databases from within the food industry. Inventory data were collected from the industry and completed using the literature and databases, impact categories were evaluated adopting a CML method with the energy analysis carried out based on the cumulative energy demand (CED).

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KROKIDA M., TAXIARCHOU M., POLITIS A., PEPPAS A., KYRIAKOPOULOU K. 2016, LIFE CYCLE ASSESSMENT (LCA) ON EUROPEAN SKIMMED MILK POWDER PROCESSING PRODUCTION PLANT. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 280-285.

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MARKET RESEARCH REGARDING THE DEMANDS OF THE BUSINESS OPERATORS ON THE SUPPLY CHAIN LOGISTICS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Mihaela Cristina DRĂGHICI, Paul Alexandru POPESCU, Adina BAICU, Mona Elena POPA

Berries are worldwide recognized as valuable source of polyphenols, especially anthocyanins, micronutrients, and fibers. They are mainly industrialized as frozen fruits but also in jams, or pies. Some berries are commercially important but there are some limitations such as: short shelf life of fresh fruit and soft texture which require special attention on the berry chain logistic. The berry industry varies from country to country as well as the types of berries cultivated or wild berries. Berries are one of the greatest assets of the Romanian forests and they are known to be products of a very high. Berries originating from non-polluted forests are very popular in the foreign markets. Unfortunately, most often they are sold in foreign markets without being locally processed and thus Romanian processors are losing valuable Romanian customers. To analyze the requirements of the industry companies (production, harvesting and distribution of native berries) a questionnaire was designed and used as a research tool. This questionnaire represents a direct tool of collecting data and to communicate with the respondents. To investigate the value chain of berry fruits and its challenges, we opted for two ways: self-administered questionnaire which was either filled in on the spot or sent by electronic mail, then filled in by the person interviewed, and the questionnaire filled in by phone which was a faster and cheaper way to obtain the necessary answers. Over 200 questionnaires were sent by email to local branches of the National Forest Administration (ROMSILVA) but only 31 were filled in and sent to us. By phone, 20 persons of interest responded to our questionnaire.

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DRĂGHICI M.C., POPESCU P.A., BAICU A., POPA M.E. 2017, MARKET RESEARCH REGARDING THE DEMANDS OF THE BUSINESS OPERATORS ON THE SUPPLY CHAIN LOGISTICS. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 260-264.


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