Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Oana LIVADARIU, Ioana-Alexandra DICU

This paper contains theoretical and practical information that has allowed the experimental setup of an effective working protocol for obtaining food sprouts in peas (Pisum sativum L.), as well as obtaining mango (Mangifera indica L.) or kiwi (Actinidia deliciosa) sorbet. In order to create a new product, mango or kiwi sorbet were combined with pea sprouts. The experimental results obtained after performing a sensory analysis of the final products, as well as an online questionnaire filled by the panelists are presented in the paper based on charts, which are demonstrating the influence of pea sprouts on consumers preferences and its purchasing behaviour.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Grigore-Mihăiță STAN, Diana CONSTANTINESCU-ARUXANDEI, Dan Florin MIHĂILESCU, Florin OANCEA

The paper aimed to identify in silico bioactive peptides with antioxidant and antihypertensive effects from silver carp collagen. This approach involved the use of a wide range of specialized online databases and tools to identify bioactive peptides from various protein sources. In this case, the collagen type-I alpha-1 protein sequence was extracted from UniProtKB with the identification code A0A077B3P8. The digestion was simulated using the BIOPEP database with the following enzymes: subtilisin, papain and pepsin. ExPASy ProtParam, Peptide Ranker, PepCalc, and ToxinPred showed that silver carp collagen is a significant source of biologically active peptides, health promoters with potential antihypertensive and antioxidant effects. The computational approach used in this study offered useful initial insights for more extensive studies.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT, Ana BUTCARU, Imad Al-SUWAID, Basim K. HASAN, Ihasn J. AZEB, Kawther ALGHASHEEM

In vitro three peach cultivars (Florin, Filip and Mimi) included in this experiment, two explants (shoots-tip and nodes) were taken at 0.5-1cm length. The explants were cultivated on 3 medium MS, B5 and QL adding of 30 g/l sucrose and 7 g/l with tested different concentrations of plant hormones BAP and NAA in 12 variants. The aim of this study was to test the effect of culture medium and growth hormones on some peach cultivars. Cultures media MS and QL showed the highest mean number of shoots at 5 mg/l BAP in (MS) medium (Florin 4.20 shoots/explant, Filip 3.60 shoots/explant and Mimi 4.00 shoots/explant respectively), and (QL) medium (Florin 4.40 shoots/explant, Filip 2.40 shoots/explant and Mimi 4.00 shoots/explant) respectively, while the (B5) medium gave the lowest value. Moreover, we were found that there is a relationship between BAP and NAA, since the addition of both hormones has led to the emergence of callus tissue, while adding just BAP to all media in the experiment gave shoots

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT, Ana BUTCARU, Imad Al-SUWAID, Basim K. HASAN, Ihasn J. AZEB, Kawther ALGHASHEEM

Three peach varieties (Florin, Filip and Mimi), two explants (shoots-tip and nodes) were tested. The explants were cultivated on 3 medium MS, B5 and QL adding of 30 g/l sucrose and 7 g/l agar without any hormone supplements during initiation stage. Cultures tubes were kept in the dark (0, 1, 2, 3, 4) days to get rid of the process of oxidation of phenolic materials resulting from cutting plant tissues. It was found during this study that the placement of the tubes of culture in the dark has a positive effect in the disposal of phenolic materials. Results presented that placing the explants in dark for 2-3 days was the best result to explants phenolic-free, in 2 days/dark (Florin 89% explant growing, Filip 83% explant growing and Mimi 85% explant growing), in 3 days/dark (Florin 89% explant growing, Filip 84% explant growing and Mimi 86% explant growing). Also QL medium give yellowish green shoots with a high resistance 95% to vitrification.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Lavinia-Diana-Nicoleta BARBU, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA, Narcisa BĂBEANU

Functional foods are gaining popularity among consumers because they contain biologically active compounds involved in human health and wellness. Although some plant species are already known for their beneficial properties if properly consumed, certain cultivation technologies can increase their content of bioactive compounds. For this purpose, plant probiotics could be applied as biostimulants, which are approved in organic agriculture. Plant probiotics are beneficial microorganisms that can be found in the rhizosphere, or as endophytes. They can be applied as single strains or as consortia. These beneficial microorganisms could increase the production of biologic active compounds in their host plants, such as: flavonoids, organic acids, phenols, tannins, and vitamins. This review focuses on various plant probiotics and their role in the bioactive compounds synthesis in their hosts. Among plant probiotics mediating bioactive compounds synthesis in plants are mentioned Azospirillum, Azotobacter, Bacillus, Phyllobacterium, Pseudomonas, Rhizobium and several other genera of plant beneficial microorganisms.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Jennifer DELACEY

WHO estimated over 700 million people were hungry in 2020, 22% of children were stunted, and nearly 30% of women were anemic. Undernutrition accounts for as much as 45% of child mortality. Global food production must increase to feed growing populations while food security is threatened by plant disease and climate change. Women and children in rural areas of low- and middle-income countries (LMICs) are the most vulnerable to undernutrition and poor health. This paper aims to review nutrition sensitive agriculture interventions for maternal and child health at the global level with a focus on the nexus of plant health and food security, and to recommend directions for future research and policy. This topic links environmental and human health within the One Health approach.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Liliana-Adriana PAIRAULT, Naomi TRITEAN, Diana CONSTANTINESCU-ARUXANDEI, Florin OANCEA

This review evaluates the technological solutions used to produce nanoformulated plant biostimulants made from biosilica recovered from silicon-rich biomass. Silicon improves root nutrient uptake/nutrient use efficiency, increases plant tolerance to stress, and promotes phytonutrients in edible crop yield. The exact mechanism of silicon action is not yet known. However, it is generally accepted that a flow of soluble silicon species through plant simplast is essential to produce the aforementioned effects. The main difficulties in applying soluble silicon species, silicic acid, and its di- and trimers to plants are related to the polycondensation features at very low concentrations. One of the solutions to this technical problem is to use amorphous silica, which constantly releases small quantities of soluble silicon species. For example, phytoliths formed in several plant species that concentrate the simplast flow of soluble silicon in their simplast are an excellent source of soluble silicon. Nanoformulation increases the surface/volume ratio and further improves the release of soluble silicon species. Our review focuses on the techniques used to extract and nanoformulate the biosilica from silicon-rich biomass.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Ramona-Daniela PAVALOIU, Fawzia SHAAT, Cristina HLEVCA, Georgeta NEAGU, Adela STARAS, Lucia PIRVU

The use of herbal cosmetics is increasing in the world market, due to the good activity with no side effects of the active ingredients from plant extracts as compared to synthetic compounds. The aim of the present study was to formulate and characterize herbal creams containing several plant extracts (Arctium lappa, Magnolia virginiana, Tiliae flos) in different combinations. The creams were prepared with different ratio of plant extracts and essential oils, while the composition of the cream base was kept the same. The characterization of the formulated creams was carried out by standard methods, for evaluating the organoleptic characteristics, physicochemical properties, microbiological contamination level and in vitro antibacterial activity against Staphylococcus aureus. Also, the plant extracts were evaluated in terms of cytotoxicity using MTS/MTT assay on L-929 fibroblasts. The creams were homogeneous, non-irritant and easily removable. The pH of creams was in the range of 5.0-5.5 which is safe for human skin. The samples were found to be populated with aerobic bacteria, yeast and fungi up to 10 CFU/g and showed moderate antibacterial activity.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Roxana-Mădălina STOICA, Angela CĂȘĂRICĂ, Ana-Despina IONESCU

Microbial exopolysaccharides (EPS) represent an important group of biologically active compounds produced and secreted by bacteria and fungi, which accumulate outside the cells. Recently, research has been focused on the exploration and discovery of new exopolysaccharides of microbial origin, due to their various biotechnological applications. Rhizobium strains produce a wide diversity of exopolysaccharides, with different structures at the species level, and a large field of applications, such as pharmaceutical, food, and cosmetics industries. In this context, this article aims to present a mini-review of the main EPS synthesized by Rhizobium strains, highlighting their structures and potential applications.

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ECO-EFFICIENCY AND TECHNO-ECONOMIC ANALYSIS OF TRICHODERMA BASED PLANT BIOSTIMULANT UTILISATION ON TOMATOES CULTIVATED IN A CONSERVATION FARMING SYSTEM

Written by Valentin ZAMFIROPOL-CRISTEA, Iulia RĂUT, Florin OANCEA
Pages 9 to 15

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ASSESSMENT OF GENETIC SIMILARITY AND PURITY DEGREE AMONG SEVERAL ROMANIAN MAIZE INBRED LINES USING SSR MARKERS

Written by Valentina VASILE, Matilda CIUCĂ, Elena NICOLAE, Cătălina VOAIDEȘ, Călina Petruța CORNEA
Pages 16 to 23

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THE GREAT POTENTIAL OF ENTOMOPHTHORALEAN FUNGI FOR BIOLOGICAL CONTROL: A REVIEW

Written by Mihaela Monica DINU, Ana-Cristina FĂTU2, Narcisa BĂBEANU
Pages 24 to 32

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ENTOMOPATHOGENIC BACTERIA VIRULENCE FACTORS AND TARGET PESTS

Written by Daniel-Nicolae COJANU, Oana-Alina BOIU-SICUIA, Ana-Maria ANDREI, Călina Petruţa CORNEA
Pages 33 to 42

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COMPARATIVE STUDY OF TWO VARIETIES OF PURPLE FLASH POTATO GROWN IN VITRO

Written by Alexandra-Mihaela NAGY, Paula BOBOC (OROS), Corina CĂTANĂ, Maria-Mihaela ANTOFIE, Camelia SAVA SAND
Pages 43 to 48

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PLANT BIOSTIMULANTS BASED ON NANOFORMULATED BIOSILICA RECOVERED FROM SILICA-RICH BIOMASS

Written by Liliana-Adriana PAIRAULT, Naomi TRITEAN, Diana CONSTANTINESCU-ARUXANDEI, Florin OANCEA
Pages 49 to 58

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IN VITRO INFLUENCE OF CULTIVARS AND DIFFERENT CULTURE MEDIA ON VITRIFICATION AND DARKNESS ON PEACH (Prunus persica L. Batsch) SHOOTS MULTIPLICATION

Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT, Ana BUTCARU, Imad Al-SUWAID, Basim K. HASAN, Ihasn J. AZEB, Kawther ALGHASHEEM
Pages 59 to 64

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DEVELOPMENT STATUS AND TREND OF PLANT FACTORY INTELLIGENCE IN CHINA

Written by Xinfa WANG, Vladislav ZUBKO, Viktor ONYCHKO, Mingfu ZHAO
Pages 65 to 70

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IN VITRO EFFECT OF CULTURE MEDIA AND GROWTH HORMONES ON THREE PEACH (Prunus persica L. Batsch) CULTIVARS

Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT, Ana BUTCARU, Imad Al-SUWAID, Basim K. HASAN, Ihasn J. AZEB, Kawther ALGHASHEEM
Pages 71 to 78

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CONVENTIONAL VERSUS MODERN TECHNIQUES USED FOR THE DETECTION OF PATHOGENS IN FOOD MATRICES: A REVIEW

Written by Quthama AL-ZAIDI, Camelia Filofteia DIGUȚĂ, Getuța DOPCEA, Florentina MATEI
Pages 79 to 88

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HIGHLIGHTING THE INFLUENCE OF PEA SPROUTS (Pisum sativum L.) ADDED TO MANGO (Mangifera indica L.) OR KIWI (Actinidia deliciosa) SORBET ON THE FINAL CONSUMER'S PURCHASING PREFERENCES

Written by Oana LIVADARIU, Ioana-Alexandra DICU
Pages 89 to 95

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THE USE OF ACID DRIED SOURDOUGH STARTER TO IMPROVE SENSORY PROPERTIES AND BREAD`S SHELF LIFE - A REVIEW

Written by Sabina-Andreea BOBEA, Nastasia BELC, Călina Petruța CORNEA
Pages 96 to 102

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BUCKWHEAT VS. SORGHUM FLOUR IN GLUTEN-FREE RICE COOKIES ENHANCED WITH PEA PROTEIN POWDER

Written by Iulia-Elena SUSMAN, Mihaela MULȚESCU, Gabriela STAMATIE, Alina CULEȚU, Mona Elena POPA
Pages 103 to 108

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STABILIZATION OF SEA BUCKTHORN (Elaeagnus rhamnoides) TURBID JUICES

Written by Mădălina GIURESCU, Luminița DIMITRIU, Florin OANCEA
Pages 109 to 116

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THE USE OF LACTIC ACID BACTERIA AND THEIR METABOLITES TO IMPROVE THE SHELF LIFE OF PERISHABLE FRUITS AND VEGETABLES

Written by Florentina BADEA, Camelia Filofteia DIGUȚĂ, Florentina MATEI
Pages 117 to 125

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IN SILICO APPROACH FOR THE IDENTIFICATION AND CHARACTERISATION OF BIOACTIVE PEPTIDES FROM SILVER CARP COLLAGEN

Written by Grigore-Mihăiță STAN, Diana CONSTANTINESCU-ARUXANDEI, Dan Florin MIHĂILESCU, Florin OANCEA
Pages 126 to 136

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BACTERIAL NANOCELLULOSE AND ITS APPLICATION FOR MUCOADHESIVE FORMULATIONS

Written by Angela MORARU, Florin OANCEA
Pages 137 to 145

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PREPARATION AND CHARACTERIZATION OF ANTIBACTERIAL CREAMS CONTAINING PLANT EXTRACTS FOR TOPICAL APPLICATION

Written by Ramona-Daniela PAVALOIU, Fawzia SHAAT, Cristina HLEVCA, Georgeta NEAGU, Adela STARAS, Lucia PIRVU
Pages 146 to 151

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HAND SANITIZERS MADE WITH NATURAL INGREDIENTS

Written by Diana GROPOŞILĂ-CONSTANTINESCU, Andreea-Antonia MILITARU, Gabriela POPA, Luminiţa VIŞAN, Gabriela MĂRGĂRIT, Dana BARBA, Radu TOMA
Pages 152 to 157

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Arctium lappa - A POTENTIAL SOURCE OF BIOACTIVE COMPOUNDS WITH PHARMACEUTICAL APPLICATIONS

Written by Ioana TABREA, Lucia PIRVU, Narcisa BĂBEANU, Călina Petruța CORNEA, Nicoleta RADU
Pages 158 to 169

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MICROBIAL BIOSTIMULANTS INCREASE BIOACTIVE COMPOUNDS IN MEDICINAL PLANTS - A REVIEW

Written by Lavinia-Diana-Nicoleta BARBU, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA, Narcisa BĂBEANU
Pages 170 to 179

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RHIZOBIAL EXOPOLYSACCHARIDES: STRUCTURE AND APPLICATIONS

Written by Roxana-Mădălina STOICA, Angela CĂȘĂRICĂ, Ana-Despina IONESCU
Pages 180 to 188

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NUTRITION SENSITIVE AGRICULTURE: FROM PLANT HEALTH TO HUMAN HEALTH

Written by Jennifer DELACEY
Pages 189 to 196

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