Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Claudia DÎRLĂ, Maria Rodica GURĂU, Fănel OȚELEA, Nicoleta DRĂGUȚ, Georgeta ȘTEFAN, Marius Andrei MĂNESCU, Doina DANEȘ

Currently, the ASF (African Swine Fever) diagnosis is carried out by the detection of viral DNA. The successful amplification of the targeted DNA fragment needs a proper quantity of the genetic material. The aim of this study was to compare the yield and quality of DNA extracted using two dedicated commercial kits. It was compared the product obtained using the Pure Link Genomic DNA Mini Kit (Invitrogen) and QIAamp Cador Pathogen Mini Kit (Qiagen). The DNA has been quantified using Qubit DNA HS Assay Kit (Qubit 3.0 Fluorometer, ThermoFisher Scientific), a highly selective over RNA and accurate tool for DNA at levels between 10 pg/µL and 100 ng/µL. The standard DNA of the ASF virus has been diluted 10_-2, 10₃, 10_-4, 10_-5 and 10_-6 as both extracts obtained with Pure Link Genomic DNA Mini Kit (Invitrogen) and the QIAamp Cador Pathogen Mini Kit (Qiagen). According to the dilutions (10_-2 to 10_-6), the DNA yield with the QIAamp Cador Pathogen Mini Kit was 132 ng/µl, 71 ng/µl, 45 ng/µl, 21.2 ng/µl and 10.2 ng/µl, respectively. The yield obtained with the Pure Link Genomic DNA Mini Kit was 126 ng/µl, 65 ng/µl, 31 ng/µl, 15.2 ng/µl and 4.2 ng/µl, respectively. The highest concentration was obtained using the QIAamp Cador Pathogen Mini Kit (Qiagen). In conclusion, the sensitivity of the Qiagen Kit is more suitable to be used for further investigation on the ASF genome.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Agnes TOMA, Oana CRACIUNESCU, Rodica TATIA, Vasile Sorin MANOIU, Florentina ISRAEL-ROMING, Gabriela LUTA, Daniela BALAN, Ştefana JURCOANE

In the present study, novel biomaterials based on mixtures of collagen and peptides isolated from aquaculture fish residues and marine chitosan solution at pH 5 were prepared, conditioned as 3D porous sponges and characterized to select best variant for biomedical applications. The physico-chemical properties of the biomaterials were investigated by determination of their in vitro biodegradability, swelling degree and porosity, while morphology was observed by scanning electron microscopy (SEM). In addition, the biocompatibility of the prepared biomaterials was assessed by cell viability of L929 fibroblast cultures cultivated in the presence of the prepared sponges by MTT assay. The results indicated that the biomaterials prepared from mixtures of collagen and chitosan at pH 5 and mixtures of collagen, chitosan at pH 5 and peptides had lower in vitro biodegradability in the presence of collagenase, higher degree of swelling and increased porosity, compared to a sponge of fish collagen (control). The cell viability values recorded in L929 fibroblast culture cultivated in the presence of the prepared biomaterials were high, indicating a good biocompatibility with skin specific cells. In conclusion, this study demonstrated that the biomaterials prepared from fish collagen and marine chitosan at pH 5 with or without addition of fish peptides have optimal physico-chemical and structural properties and good biocompatibility, recommending their further testing and use in skin tissue engineering.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Mirela DRĂGAN, Andrei PETRE, Silvana Mihaela DĂNĂILĂ-GUIDEA, Ștefana JURCOANE

The general objective of the research work was to identify species of plant crops with honeydew potential during the July-October period when bee food is reduced, and it is necessary to supplement the bee family with artificial food. After harvesting the main commercial crops (barley, oats, wheat), honey plants were cultivated on the respective plots: buckwheat, mustard, phacelia, peas and camelina, which bloom during this period. After flowering, all these crops were incorporated into the soil by mechanized plowing in order to increase the amount of humus and keep the soil green, thus ensuring an ecological method of sustainability (cover crops). To determine the honeydew capacity of the selected crops, the capillary method was applied throughout the flowering period: or 3 repetitions were performed for each crop during the period of maximum nectar accumulation capacity, indicated by the specialized literature.

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ENHANCING STORAGE CAPACITY OF POTATO SYNTHETIC SEEDS THROUGH THE USE OF SALICYLIC ACID

Written by Ioana-Cătălina NICOLAE, Oana VENAT, Adrian George PETICILĂ, Dorel HOZA
Pages 7 to 15

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IN VIVO TESTING OF LACTIC ACID BACTERIA AS BIOCONTROL AGENTS AGAINST B. cinerea ON WHITE GRAPE BERRIES

Written by Florentina BADEA, Constantin Alexandru ALDEA, Camelia Filofteia DIGUȚĂ, Florentina MATEI
Pages 16 to 23

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ADVANCES IN ANTIMICROBIAL CONTROL: CONTRIBUTIONS AND POTENTIAL APPLICATIONS OF ANTAGONISTIC BACTERIA IN THE CONTROL OF MULTIDRUG-RESISTANT PATHOGENIC BACTERIA

Written by Cheik Omar Tidiane COMPAORÉ, Catalina Mihaela VOAIDES, Iliassou MOGMENGA, Amidou S. OUILI, Franck MOSSOUN, Ynoussa MAIGA, Aboubakar Sidiki OUATTARA
Pages 24 to 35

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SENSORY ANALYSIS EVALUATION STUDY OF FOREIGN AND NATIONAL APRICOT VARIETIES

Written by Imad Al-SUWAID, Cosmin MIHAI, Ana BUTCARU, Florin STĂNICĂ
Pages 36 to 42

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COMPARATIVE ANALYSIS OF BRAZZEIN PRODUCTION IN IN VIVO AND IN VITRO SYSTEMS

Written by Ioannis Ilie KARPATHAKIS, Călina Petruţa CORNEA
Pages 43 to 49

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VALORIZATION OF VEGETAL BY-PRODUCTS IN NEW MATRICES FOR THE IMPROVED FOOD SUSTAINABILITY SYSTEMS

Written by Adina NICHITA, Mona Elena POPA
Pages 50 to 58

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HEAVY METAL CONTAMINATION IN SOIL AND ITS ACCUMULATION IN HOME GROWN TOMATO (Solanum lycopersicum L.) FRUITS

Written by Natália ČERYOVÁ, Judita LIDIKOVÁ, Monika ŇORBOVÁ, Silvia FEDORKOVÁ, Alena VOLLMANNOVÁ, Janette MUSILOVÁ, Ľuboš HARANGOZO
Pages 59 to 65

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TESTING THE FERMENTATIVE POTENTIAL OF SOME LOCAL Saccharomyces AND non-Saccharomyces YEASTS

Written by Raluca-Ștefania RĂDOI-ENCEA, Florentina BADEA, Ana-Maria MANOLICĂ, Luminița VIȘAN, Florentina MATEI
Pages 66 to 73

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LIPOLYTIC AND CUTINOLYTIC ACTIVITY OF MICROORGANISMS ISOLATED FROM POLYETHYLENE SURFACE

Written by Olga POSTOLACHI, Inna RASTIMESINA, Vera MAMALIGA
Pages 74 to 80

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BIODEGRADATION OF LIGNOCELLULOSIC SUBSTRATES WITH IMPROVED FUNGAL STRAINS

Written by Aglaia POPA
Pages 81 to 86

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ASSESSMENT OF GRAPES INDIGENOUS MICROBIOME FROM “ȘTEFAN VODĂ” PROTECTED GEOGRAPHICAL INDICATION

Written by Natalia VLADEI, Ecaterina COVACI, Alexandra ARSENI, Valeria DAMASCHIN
Pages 87 to 94

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SCREENING OF MICROBIAL STRAINS ABLE TO PRODUCE EXTRACELLULAR LIPASES

Written by Oana GHERGHESCU, Angela CĂŞĂRICĂ, Mișu MOSCOVICI, Florentina ISRAEL-ROMING
Pages 95 to 100

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INFLUENCE OF MEMBRANE SEPARATION TECHNIQUE UPON THE PHENOLIC CONTENT OF RED CORN BRAN EXTRACT

Written by Andrei Constantin Ioan ARLET, Brindusa BALANUCA, Mihaela TOCIU, Cristina OTT, Lucian CONSTANTIN, Aglaia POPA, Florentina ISRAEL-ROMING
Pages 101 to 109

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PERFORMANCES OF TWO EXTRACTION KITS OF AFRICAN SWINE VIRUS GENOME

Written by Claudia DÎRLĂ, Maria Rodica GURĂU, Fănel OȚELEA, Nicoleta DRĂGUȚ, Georgeta ȘTEFAN, Marius Andrei MĂNESCU, Doina DANEȘ
Pages 110 to 115

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PREPARATION AND CHARACTERIZATION OF NOVEL BIOMATERIALS BASED ON MIXTURES OF FISH COLLAGEN AND PEPTIDES WITH CHITOSAN FOR BIOMEDICAL APPLICATIONS

Written by Agnes TOMA, Oana CRACIUNESCU, Rodica TATIA, Vasile Sorin MANOIU, Florentina ISRAEL-ROMING, Gabriela LUTA, Daniela BALAN, Ştefana JURCOANE
Pages 116 to 121

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TOXIGENIC MOLDS CONTAMINANTS IN SUDANESE POULTRY FEED AND THEIR POTENTIAL BIOCONTROL

Written by Asawir Esamaldeen Ebrahim MOHAMED, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA
Pages 122 to 127

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RESEARCH INTO INCREASE BEE PRODUCTIVITY USING COVER CROPS

Written by Mirela DRĂGAN, Andrei PETRE, Silvana Mihaela DĂNĂILĂ-GUIDEA, Ștefana JURCOANE
Pages 128 to 133

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THEORETICAL EXPLORING OF ANTIGENOTOXIC PLANT INGREDIENTS WITH ANTIAGING PROPERTIES

Written by Petronela MUNTEANU, Adina NICHITA, Florentina Vinica BARBU, Laura FEODOROV, Laura-Dorina DINU, Emanuel VAMANU
Pages 134 to 140

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FOOD CONTAMINANTS INCIDENCE ON CEREAL VALUE CHAIN, A MINIREVIEW

Written by Mihaela VILCU, Elisabeta Elena POPA, Mona Elena POPA
Pages 141 to 146

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THE RESULTS OF AN ACADEMIC EXERCISE OF USING AI AS AN TOOL FOR IDENTIFYING CERTAIN KEYWORDS IN USAMVB SCIENTIFIC ARTICLES

Written by Oana LIVADARIU, Alexandra ADAM, Ana ENE
Pages 147 to 154

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UNVEILING THE FUNCTIONAL PROPERTIES OF Pelargonium graveolens: INSIGHTS INTO ITS BIOLOGICAL ACTIVITIES

Written by Sara ROMAN, Cătălina VOAIDEȘ, Narcisa-Elena BĂBEANU
Pages 155 to 162

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Oana GHERGHESCU, Angela CĂŞĂRICĂ, Mișu MOSCOVICI, Florentina ISRAEL-ROMING

Lipases have gained attention worldwide, due to their potential for diverse applications and their stability and selectivity. The study’s aim was to identify microorganisms with the ability to synthesize extracellular lipase. In order to achieve this, 20 microbial strains were cultivated on selective solid agar media and lipase producing microorganisms were selected based upon their ratio of the diameter of the halo (if present) and the colony’s diameter. The bacterial strains were cultivated on three different selective media, the results indicating that the best screening medium was TBA and the strain that was estimated to have the highest lipase activity was Bacillus subtilis ICCF 20. Yeast strains were cultivated on two selective media and M4D was selected as the best screening medium. The highest producer of lipases was considered to be Yarrowia lipolytica ATCC 16618 ICCF 214. The fungal strains were cultivated on two selective media and the best screening medium was determined to be YS. Two fungal strains were selected as having the highest lipase activity: Aspergillus niger (P4 C36) ICCF 24 and Aspergillus awamory (P2 C114) ICCF 259.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Imad Al-SUWAID, Cosmin MIHAI, Ana BUTCARU, Florin STĂNICĂ

This research aimed to investigate the significant consumer indicators in apricot fruit by different groups of Romanian and foreign cultivars. The sensory analysis was carried out in three harvest seasons during 2021-2023, and the method used was questionnaires (tasting sheets). The analyzed parameters were: the appearance of the fruit given the size (1-3), form (1-3), color (1-3); pulp firmness (1-4), pulp juiciness (1-5), taste (1-6), and flavor (1-4). The evaluation of the size and shape of the fruits in the Romanian and foreign cultivars showed that in both batches, in general, the fruits had very good ratings and were very well appreciated. The fruit color index varied between 2 and 3 in both studied groups. Pulp firmness analysis showed that the uniformity of this index was higher in the foreign cultivars. Examining fruit taste and pulp juiciness showed that the fruits of both groups were almost in the same range and had similar grades. The best taste among the foreign cultivars was observed in Pisina/M29C and among the Romanian cultivars in Amiral and Orizont.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Raluca-Ștefania RĂDOI-ENCEA, Florentina BADEA, Ana-Maria MANOLICĂ, Luminița VIȘAN, Florentina MATEI

Considering that Starmerella bacillaris and Metschnikowia pulcherrima have repeatedly proved their desirable oenological properties, we have chosen to test the fermentative potential of local strains from our collection, in simple and sequential cultures with a local Saccharomyces cerevisiae. In order to prove their winemaking potential, the fermentative kinetics were observed, along with ethanol and sugar content, pH, total dry matter and wines colors. Local yeast S. cerevisiae BB06 finalized the fermentation with 10.5-11% ethanol and 9.5-9.3°Bx. The two sequential fermentations with S. bacillaris MI115 and M. pulcherrima MI109 led to an average ethanol content of 7.8%, respectively 7.8% and around 13.9°Bx, respectively 16.6°Bx. The pH levels were maintained between 3.0 and 4.0 throughout the experiments. The three tested strains confirmed they are suitable for winemaking in sequential steps, but several further investigations should be performed on biochemical and organoleptic level.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Oana LIVADARIU, Alexandra ADAM, Ana ENE

This article presents the results of an academic exercise aimed at selecting an AI-efficient working tool between AISA1 from TC1 and AISA2 from TC2 to identify LED’s light, and plant keywords in scientific articles available on-line from publications of USAMV of Bucharest. Such a tool is necessary for students, teachers, and researchers who have scientific activity with implications in the use of LED’s light to plants. Each tool used three keyword search variants, namely: individual keywords, a succession of keywords, and an explicit request for identification (identifies in the scientific publications of USAMV of Bucharest the following keywords: LED’s, light, and plants). The results obtained, for each of the three variants of keyword identification, indicate both the use of the AISA1 AI-based working tool from TC1 for quick identification of text-based information without identification by accurate academic citation of the source, as well as using the AISA2 AI-based tool from TC2 to quickly identify text information and sources as links that it must be accessed, studied and selected in established academic mode. Both tools can provide information that through concatenation generates a basic image for the primary verification of the existence of LED’s, light, and plant keywords, in scientific articles available on-line from publications of USAMV of Bucharest.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Petronela MUNTEANU, Adina NICHITA, Florentina Vinica BARBU, Laura FEODOROV, Laura-Dorina DINU, Emanuel VAMANU

Aging could be considered a chronic disorder that comes with more devastating traits than we could imagine or see, both at cellular and molecular levels. The paper aimed to summarize the molecular mechanisms of ageing and to emphasize some plants which have antigenotoxic properties that can be linked to antiaging features. In cosmeceutical manufacturing, using natural and organic ingredients is the best way to maintain and offer skin rejuvenation qualities. Moreover, the synergy between plant phytochemicals can enhance the overall antiaging effect, addressing multiple aspects of skin aging simultaneously. There are tremendous antigenotoxic plants provided by nature, while few of them have antiaging features, such as: Trichilia emetica, Psoralea corylifolia, Ximenia americana, Schinziophyton rautanenii, and Hypericum perforatum. Thus, the review outlined their benefits in order to reveal their therapeutic potential in the cosmetic industry.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Asawir Esamaldeen Ebrahim MOHAMED, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA

This study focuses on Penicillium and similar other mold contaminants found in poultry feed sourced from Sudan. Such fungi were found in sorghum kernels, shelled peanuts, wheat bran and peanut meal from seven Sudanese regions. These fungi were isolated and characterized based on their microscopic features and colony morphology on Dicloran Rose Bengal Chloramphenicol Agar (DRBC), Potato Dextrose Agar (PDA), and Pentachloronitrobenzene - Rose Bengal - Yeast extract - Sucrose Agar (PRYES). To evaluate their mycotoxigenic potential, chromatographic and genetic approaches were used. Fungal extracts were evaluated for mycotoxins content by Thin-Layer Chromatography (TLC). Structural and regulatory genes such as nor-1, ver-1, omt-A, aflR, ota.nps and patN, involved in mycotoxins production were analyzed by classical PCR technology. The strains were also identified by sequencing the ITS1 – 5,8S – ITS2 region. To inhibit the growth of Penicillium isolate certain biocontrol agents were used, revealing good antifungal potential. These findings provide valuable insights into the nature of Penicillium contaminants in Sudanese poultry feed, providing fundamental knowledge for further research on managing mycotoxigenic contaminants and feed safety.

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