BENEFITS OF THE E-LEARNING PLATFORMS AND CLOUD COMPUTING IN THE BIOTECHNOLOGY EDUCATION

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Radu Cristian TOMA, Gabriela MARGARIT, Diana GROPOSILA, Dana BARBA

Online education represents a new approach of learning process, in which substantive elements remain the same, only the means of exchange of knowledge and learning is different. Few benefits of the e-learning platforms and cloud computing in educational area are: a reduced infrastructure and IT costs, an increased accessibility, a better collaboration, and allow organizations more flexibility. But cloud computing is having other effects as well, which have the potential to greatly change how education works, both in online and offline (traditional classrooms) courses like: no more expensive textbooks, no more outdated learning materials, no expensive hardware and software required, reaching more diverse students.

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TOMA R.C., MARGARIT G., GROPOSILA D., BARBA D. 2016, BENEFITS OF THE E-LEARNING PLATFORMS AND CLOUD COMPUTING IN THE BIOTECHNOLOGY EDUCATION. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 370-373.

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BINDERING AND THE STABILITY OF BETA CAROTEN FROM Neurospora sitophila

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Jhondri JHONDRI, Abun ABUN, Denny RUSMANA, Rachmat WIRADIMADJA, Handi BURHANUDDI

Neurospora sitophila, a species of fermetation fungi was reported containing the β-carotene pigments, the secondary metabolite of the yellow, orange or red-orange pigments groups, On this research, the carotenoid pigments of Neurospora sitophila has been bindered by a Oligosacharide of gelatin-maltodextrins. The bindered products were dried by a spray drier and the β-carotene powder was determined its stability to the storage influence at Relative Humidity 20-30%. Bindered product of β-carotene extract obtained the GME powder with BY value±50 %. Bindered of this extract β- carotene was increased the water solubility and stable at Relative Humidity 20-30% condition, the stability the powder (GME) was decreased start at the third week. HPLC analysis of GME powder showed the decreasing of β-carotene about 30%, after storage for 5 weeks.

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JHONDRI J., ABUN A., RUSMANA D., WIRADIMADJA R., BURHANUDDI H. 2017, BINDERING AND THE STABILITY OF BETA CAROTEN FROM Neurospora sitophila. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 143-148.

CAMELINA SATIVA OIL-A REVIEW

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Alina-Loredana POPA, Ștefana JURCOANE, Brândușa DUMITRIU

Camelina sativa is an oil seeded plant belonging to the Brassicaceae family. It can be cultivated both in winter and spring season, having a remarkable capacity to adapt and resist to difficult climate conditions. Moreover, Camelina crop has shown resistance to pests and diseases which affect other crops from the same family. The synthesis of phytoalexins seems to be responsible for the unusual camelina defense system. Camelina oil is the main product resulted by extraction from seeds. The most common extraction methods are: mechanical extraction, solvent extraction and enzymatic extraction. Recently it has been considered also the supercritical-CO2 extraction. The oil obtained contains an unsaponifiable fraction represented by tocopherols, sterols and a saponifiable fraction consisting in fatty acids. The fatty acids profile is mainly represented by unsaturated fatty acids- mono and mostly polyunsaturated (>55%) and saturated fatty acids (9.1-10.8%). The most frequent fatty acids from camelina oil are linolenic, linoleic, oleic and eicosenoic. In comparison with other Brassicaceae plants, camelina oil has a low content of erucic acid. Camelina oil, due to its composition, has multiple uses in various industries: feed technology for substitution or supplementation of other oils (fish, broilers) in animal diets, biodiesel production, jet fuel production, biopolymer industry (peel adhesion properties, paints, varnishes), cosmetic industry (skin-conditioning agent), in food products due to its high omega-3 fatty acid content and low erucic acid content and as milk fat substitution.

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POPA A.L., JURCOANE S., DUMITRIU B. 2017, CAMELINA SATIVA OIL-A REVIEW. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 233-238.

COLOR CHANGES DURING THE STORAGE IN BOTTLES OF MUSCAT OTTONEL WINE TREATED WITH ASCORBIC ACID AND GLUTATHIONE

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Gianina Antonela BADEA, Arina Oana ANTOCE

The present work aimed to determine the colour evolution after aging in bottle of a white wine from Muscat Ottonel cultivar and the effect of the addition of different antioxidants at bottling time, such as SO2, reduced glutathione (GSH) and ascorbic acid. Wine colour is an important indicator of its degree of oxidation, therefore the evolution toward a brownish colour is equivalent with some quality loss or even degradation. Considering that colour is also a key parameter that contributes to the perception of the quality profile of a white wine, the study attempted to determine the effect of certain doses and combinations of some anti-oxidants, which are known to ensure the best wine quality preservation during aging of wine in bottles. Our preliminary study shows that, in some cases and conditions, the antioxidants used to protect the aroma and the colour of wines end up affecting the colour.

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BADEA G.A.,ANTOCE A.O. 2016, COLOR CHANGES DURING THE STORAGE IN BOTTLES OF MUSCAT OTTONEL WINE TREATED WITH ASCORBIC ACID AND GLUTATHIONE. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 121-128.

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COMPARISON OF THREE DETECTION METHODS OF PHYTOPLASMA AT APPLE TREES PROVES THE ADVANTAGE OF AMPLIFICATION OF SPECIFIC 16SrADN

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Desareda MERO, Ariola BACU, Margarita HYSKO

During 2015, samples from 30 apple trees growing in three plantations of Korca were tested for phytoplasmic infections. The last have been difficult to detect due to their low concentration especially in woody hosts and their erratic distribution in the sieve tubes of the infected plants. After the evaluation of the sanitary status of three apple collections in Korca district based on classical symptomatology and DAPI staining method, in order to give a final assessment, one 16SrADN sequence of phytoplasma genome was amplified from DNA extracted from roots, stems and trunks. Quality of DNA is of key importance in molecular diagnostics, since it can affect the final result. It depends on sampling material and which plant tissue is examined. Results were used to identify the infected tissues. Results obtained by classical, cytological and molecular methods, were compared to understand the sanitary status of collections and to compare the effectiveness of each of them.

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MERO D., BACU A., HYSKO M. 2016, COMPARISON OF THREE DETECTION METHODS OF PHYTOPLASMA AT APPLE TREES PROVES THE ADVANTAGE OF AMPLIFICATION OF SPECIFIC 16SrADN. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 61-64.

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CONTAMINATION OF POULTRY FEED BY POTENTIALLY TOXIGENIC FUNGI

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Vesna KRNJAJA, Aleksandar STANOJKOVIĆ, Zorica BIJELIĆ, Violeta MANDIĆ, Miloš LUKIĆ, Zdenka ŠKRBIĆ, Veselin PETRIČEVIĆ

This study examines the mycological quality of poultry feed in 69 samples (45 samples of chicken feed and 24 samples of feed for layers) in 2014 and in 44 samples of poultry feed (34 samples of chicken feed and 10 samples of feed for layers) in 2015. The total fungal count was determined using a dilution method, and standard mycological methods were used to identify potential toxigenic fungi genera. The chicken feed contained total fungal count above the allowed limit in 26.67% of samples in 2014, and in 14.71% of samples in 2015, while the total fungal count above the limit in the feed for layers was established only in year 2014, in 8.33% of the samples. Potentially toxigenic fungi species belonging to the genera Aspergillus, Fusarium and Penicillium were identified in both groups of studied poultry feed mixtures during both years. In chicken feed, the highest number of Fusarium positive samples in both years was recorded, 73.3% in 2014 and 64.71% of the samples in 2015. In the feed for layers, in the majority of samples (83.33%), Fusarium species were identified in 2014, while in year 2015, the species of the genera Aspergillus and Fusarium were identified in the majority of samples (90%). Based on these results it can be concluded that the sanitary and hygiene conditions during the production of poultry feed must be determined by specific strategies for the reduction of the incidence of potentially toxigenic species of the genera Aspergillus, Fusarium and Penicillium. This strategy involves the use of complex and integrated measures to combat, especially during the growing of grain and other plants as the main raw materials, as well as during periods of storage and preservation of basic raw materials and finished mixtures used for poultry feeding.

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KRNJAJA V., STANOJKOVIĆ A., BIJELIĆ Z., MANDIĆ V., LUKIĆ M., ŠKRBIĆ Z., PETRIČEVIĆ V. 2017, CONTAMINATION OF POULTRY FEED BY POTENTIALLY TOXIGENIC FUNGI. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 270-274.

CRITICAL REVIEW ON PROCESSING EFFECT ON NUTRITIONAL COMPOSITION OF FOOD PRODUCTS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Georgiana-Aurora ŞTEFÃNOIU, Elisabeta Elena POPA, Amalia Carmen MITELUŢ, Mona Elena POPA

An increase in the intake of food and constant changes in the formulation of food products has become evident in recent years. Therefore the efforts of processors together with academic circles in attending consumer demands for high quality food and dealing with raising economic standards, and nowadays particularly with ecological concerns, has triggered the development of emergent technological approaches for food processing. Recently, unconventional treatments technologies in food processing have gained increased industrial interest and have potential to replace, at least partially, the traditional well-established preservation processes (Pereira and Vicente, 2010). The objective of this work is to make a short review in respect to unconventional treatments of food, which are used nowadays in industry or are in the research and development phase. The paper presents the effects of novel techniques on the quality of food products, especially on the nutritional value.

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ŞTEFÃNOIU G.A., POPA E.E., MITELUŢ A.C., POPA M.E. 2017, CRITICAL REVIEW ON PROCESSING EFFECT ON NUTRITIONAL COMPOSITION OF FOOD PRODUCTS. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 249-259.

CULTIVATION OF FLORIDA OYSTER MUSHROOM ON VARIOUS TYPES OF SUBSTRATE

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Denisa STĂNESCU, Emanuel VAMANU

Valorisation of agricultural wastes is one of the main objectives for activity optimization in agriculture industry. One method for waste utilization implies their use as substrate for obtaining edible mushrooms, which are a raw material of interest in current food industry. The aim of the paper was the cultivation of Pleurotus ostreatus var. florida species, on diverse common plant wastes. The species had good fructification, resulting numerous pins on the surface of the substrate, the cap diameter reaching a maximum of 4 to 5 cm. The color of the basidium was brighter, because the cultivation temperatures were above 20°C most of the time. The fructification time decreased with the increase of inoculation rate. Although major differences haven’t been determined for morphological characteristics of the fructification body, the supplementation of the substrate formula with other components determined approximately 10% increase in productivity. The study proved that applying supplements to the substrate formula lead to the optimization of valorisation of plant wastes taken into consideration. Also, the use of supplements did not stimulate infection rate of the substrate.

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STĂNESCU D., VAMANU E. 2016, CULTIVATION OF FLORIDA OYSTER MUSHROOM ON VARIOUS TYPES OF SUBSTRATE. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 374-378.

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DETECTION OF GENETIC VARIABILITY IN Pleurotus eryngii USING TUBULIN-BASED POLYMORPHISM MOLECULAR MARKERS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Gabriela POPA, Catalina VOAIDES, Matilda CIUCA, Calina Petruta CORNEA

Pleurotus eryngii is an edible mushroom with important biological functions, such as anticancer, antiviral, immune potentiating, and hypolipidemic activities. Due to its importance from economically, medicinal and ecologically point of view, a special attention was given to this species worldwide. Analyses via molecular markers revealed that Pleurotuseryngii is a species complex comprises at least six varieties (natural isolates and commercial strains). Improvement of culture technologies as well as the increase of biological compounds content involves a better knowledge of the genetic properties of each isolate, candidate for specific applications. For this reason, in this study several strains of Pleurotus eryngii collected from different geographical locations were analyzed in correlation with a wild Pleurotus ostreatus isolate, in order to determine the genetic variability and relationship between species. The genetic diversity of biological material was analyzed using a TBP (tubulin-based polymorphism) molecular marker that relies on the presence of intron-specific DNA polymorphisms of the plant β-tubulin gene family. For the fungal DNA extraction mycelium obtained on plates with PDA was used. Our results revealed that TBP amplification profile of intron 1 showed eight polymorphic bands while TBP amplification profile of intron 2 indicated nine polymorphic bands.The multiple length polymorphism of β-tubulin intron 2 generated amplification profiles which resulted highly specific for each of the analyzed species. However, these results suggested that TBP molecular markers could be used for highlighting the genetic diversity both intra- and inter-specific and the genetic diversity was closely related to the geographical distribution of this fungus.

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POPA G., VOAIDES C., CIUCA M., CORNEA C.P. 2016, DETECTION OF GENETIC VARIABILITY IN Pleurotus eryngii USING TUBULIN-BASED POLYMORPHISM MOLECULAR MARKERS. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 162-166.

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DOWNY MILDEW IN SUNFLOWER - THE MANAGEMENT OF Plasmopara halstedii PATHOGEN

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Gabriel Florin ANTON, Maria JOIȚA-PĂCUREANU, Luxiţa RÎŞNOVEANU, Călina Petruţa CORNEA, Mihaela POPA

The virulence of the downy mildew pathogen increased during the last period and new virulent races of this fungus appear. Thus it is essential to know data about the presence and distribution of the pathotypes. In Europe, an increasing number of the pathotypes, have been identified. In Romania we identified eight races of the pathogen. They are not present in all areas cultivated with sunflower, in some areas being present only five races, in other six or seven. Using the sources of resistance to Plasmopoara halstedii pathogen, for the most virulent races present now in Europe we have introduced genes of resistance in the best lines from our germplasm collection. The testing of some sunflower hybrids with different degree of resistance to downy mildew has showed that there is difference in the pathogen attack degree, depending on the climatic conditions in the years and locations.

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ANTON G.F., JOIȚA-PĂCUREANU M., RÎŞNOVEANU L., CORNEA C.P., POPA M. 2017, DOWNY MILDEW IN SUNFLOWER - THE MANAGEMENT OF Plasmopara halstedii PATHOGEN. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 29-32.


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