ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364
 

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Ana-Valentina ARDELEAN, Călina Petruţa CORNEA, Cristina MOISESCU, Ioan ARDELEAN

Photosynthetic microorganisms, in addition to the major planetary role in solar energy conversion and biogeochemical cycles, have a major contribution to a wide range of biotechnological applications. In this paper, we present our original results concerning the use of different concentrations of nitrate as stressor for enhancing the lipid content in our isolate, a green microalga named Rd. The effects on cellular growth, lipid, total protein, chlorophyll a and b, and carotenes content are also presented. The results are discussed with respect to applicative and basic research approaches for a deeper understanding of the mechanisms involved in the response of microorganisms to stress.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Mariana Valentina PETRE, Mona Elena POPA

Wheat is one of the most important cereals grown worldwide. After harvesting it must be stored in appropriate conditions in order to retains its nutritional and quality properties. During storage the wheat can be degraded due to improper storage conditions. The analyzes that are assessed to establish the quality of the wheat stored for a longer period are: proteins content, humidity, ash, Falling Number test and aflatoxins incidence. This paper makes an overview of the storage conditions available worldwide, the effects of the storage conditions on the quality of the wheat preserved in silos, warehouses or halls. In order to monitor the wheat storage condition it is necessary to follow the important parameters of storage, temperature and relative humidity. Optimal storage can ensure the good quality and and ventilation. Therefore, it is very important that the storage areas are properly prepared after harvesting. This preparation involves a general cleaning, followed by disinfection, derating and repair of cracks in the walls and floor, which, could become nests conducive to the development of insect larvae. Proper storage of agricultural products but also initial quality parameters of raw material represent very important factors for maintaining their quality and quantity for a long period of time.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Elena MIHAI (DRĂGHICI), Ramona-Daniela PAVALOIU, Fawzia SHA'AT, Maria PELE

Peanut allergy is one of the most common food allergies. Peanuts can cause a severe, potentially life-threatening allergic reaction (anaphylaxis). In order to improve consumer protection, our study is geared to quantification of proteins through several extraction methods. Fried peanuts samples were firstly defatted by stirring with pre-cooled acetone and 0.07% β-mercaptoethanol and then extracted with different buffers. The effect of various extraction procedures on the extraction efficiency for peanut protein was investigated by Lowry assay. Bovine serum albumin (BSA) was used as standard. Our work shows that the results depend on extraction method used.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Mihaela DUMITRU, Mihaela HĂBEANU, Nicoleta LEFTER

Direct-fed microbial (DFM) supplementation in piglet’s nutrition may offer high benefits to the young animals by diminishing the bacteria pathogens from the gastrointestinal tract and environment. In this study, we evaluated the efficiency of Bacillus subtilis ATCC 6051a (BS, 1.6 x 109 CFU/mL) on the piglet’s microbiota in the weaning crisis. A total of 60 piglets, 30 days ± 3 days of age, were allotted in 3 homogeneous groups (C, E1 and E2, 10 piglets/6 pens, 2 replicates/group) supplemented with BS 1% (E1), respectively 3% (E2)/kg feed. At the end of the trial (46 ± 3 d-old), 6 piglets were slaughtered (2 piglets/group) and intestinal content (ileum and cecum) were collected for evaluating the microbiota and intestinal pH values. The piglet’s faces were collected for microbial analysis on 1-d, 8d, and 16 d. The numbers of lactic acid bacteria (LAB), Coliforms bacteria, Enterococcus spp., Clostridium spp. and Bacillus spp. from intestinal content were modified at the addition of BS, whereas, from faeces samples, the microbiota was insignificant (P ≥ 0.05). The addition of BS 1% and 3% decreased the numbers of Escherichia coli biotype β-hemolytic from piglet’s intestinal content (P ≤ 0.05) and faces vs. C group. Salmonella spp. was not present. The intestinal pH from the ileum and cecum segment was observed to be lower in E1 + BS 1%, while in E2 + BS 3% the pH was higher vs. C group. In conclusion, 1% supplementation of Bacillus subtilis ATCC 6051a in piglets feed had a positive effect during the postweaning period on endogenous microbiota, fecal microbial count and intestinal pH evolution.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Indira GALIT, Alexandru Cristian GROSU, Narcisa BĂBEANU, Ovidiu POPA

The watermelon is a fruit used on a large scale in human nutrition. Cucurbitceae species are a source of many nutrients such as protein,minerals, lipids as well as ingredients for medicine. The main non-edible parts are the seeds and the rind that make them agro-wastes. Watermelon seeds and rind may represent an appropiate tool for the treatment of various diseases in those countries that use a more classical therapeutic approach. In this article is made a brief analysis of the ways to recover the resulted wastes from the cultivation of watermelon. The most promising directions are obtaining the bioactive compounds, biofertilizers and biogas.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Silvana Mihaela DĂNĂILĂ-GUIDEA, Gabriela NEAȚĂ, Floarea BURNICHI, Paul-Alexandru POPESCU, Mihaela DRĂGHICI, Elisabeta Elena POPA, Alexandru-Alin SANDA, Alexandra ŞOGOR, Ricuța-Vasilica DOBRINOIU, Valerica-Luminița VIȘAN, Gabriela Lucica MĂRGĂRIT, Mihaela GEICU-CRISTEA, Amalia Carmen MITELUŢ, Mona Elena POPA

The aim of our research was to establish the effect of some cultivation stages in ecological system in which are use the LED light on biological material of three Romanian tomato varieties. For this purpose, young tomatoes (Lycopersicon esculentum Mill.) plants have been grown in protected crop space (vegetation house), by daily supplementing natural light for short periods of time with light provided by high power LED panels with red, blue and white monochromatic light. In order to carry out the integrated studies of the cultivation method in the ecological system, products accepted as natural and biodegradable and approved in Romania, for fertilization and control of the attacks of diseases and pests, were used . The tested varieties were selected with indefinite habitus and special forms of fruits, namely: Sonia de Buzău, Hera and Coralina. The recorded differences in the increase of the stems' length (average values) recorded, after 50 days from the beginning of the experiments, have revealed to us that the results are differentiated according to genotype, spectrum and exposure time to the used additional light. Plants of the variety “Sonia de Buzău” exposed daily under LED light recorded high values in most variants, followed by those of the variety Hera and Coralina. The results obtained after the application of additional treatments with LED light, were dynamically analysed, evaluated by statistical calculation and compared with the values obtained by the Control plants maintained only under the effect of natural light and which were treated with the fertilization and phytosanitary protection products.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Elena COARFĂ, Mona Elena POPA

The acidity of grapevine products has a major influence on several aspects. It is involved in the microbiological, tartan and protein stability, determines the color of wine and the speed of maturation and ageing, but in particular, determines the taste balance. This paper aimed to present the evolution of content in organic acids during the period 2017-2019 in the Valea Călugărească vineyard from three grapes cultivars: Cabernet Sauvignon, Merlot and Fetească Neagră which were analysed through Ion Chromatography (IC-MS) method. The experimental measurements established that the ripe grapes and the must contains in the beginning higher amounts of malic acid: for example in 2017, 1120 mg/l malic acid in November and only 324 mg/l in March 2018 comparing with next harvest when the malic acid content was in November 2018 - 1389 mg/l and decreased to 310 mg/l in March 2019. In terms of ecoclimatic conditions 2017 was characterized by a moderate heliothermal regime, especially in April and May, when the average annual values were overcome compared to 2018 when the heliothermic regime was high and based on relatively low water resources.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Georgia OLARU, Maria RAPA, Mona Elena POPA

Biopolymers are polymers obtained from biological origins and used for industrial applications in food packaging. A biopolymer should be non-toxic, non-carcinogenic, biodegradable and also adequately available for their widespread applications. The aim of this study was to obtain packaging materials for food, as films prepared in the laboratory, based on natural polymers obtained from renewable resources: - chitosan/gelatine and chitosan/gelatine/n-clay respectively; - chitosan/pectin and chitosan/pectin/n-clay respectively; - chitosan/gelatine/pectin and chitosan/gelatine/pectin/n-clay respectively. The nano-clay was added 3% to the mixing mass. In order to obtain films with a good flexibility, 5% glycerol was added. These polymeric films have been characterized in terms of transmittance/transmission by UV/VIS spectroscopy and chemical structure by ATR-FTIR technique. It was found that the optical properties of these films were slightly affected by the nano-clay concentration in their composition. As for the films based on chitosan - gelatine, chitosan-pectin, which contain nano-clay, this has increased the thickness of the films, giving them mechanical strength, but also opacity. Analysis of the film microstructure obtained by FTIR exhibited a good compatibility among the gelatine-chitosan blend and showed that this matrix allowed a uniform distribution of the actives throughout the network. The results obtained from the ATR-FTIR spectra confirmed the existence of specific chemical groups in the analysed polymers (-OH, -NH2, -NH, CH2, CH- groups), according to the data reported in the literature.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Indira GALIT, Alexandru Cristian GROSU, Ovidiu POPA, Narcisa BĂBEANU

Oil extracts from a number of many fruits, nuts and seeds are being used in soap making, cooking, pharmaceutical products and other industries. The seeds of numerous fruits are thrown out as waste despite of their nutritional values. Many of them represent an important source of high quality protein and vitamins. Watermelon seed oil has a large concentration of unsaturated fatty acids. The low acid, peroxide and iodine values of the oil together with some favourable functional properties makes the watermelon seed and seed oil suitable for human use rather than as a waste where the crop is grown in abundance. The study was realized using Soxhlet method and micro-Kjeldahl. Citrullus lanatus seed oil has a lot of nutritional compounds. As mineral content is shown that is rich in phosphorus 30.8 mg, magnesium 2.98 mg and sodium 2.3 mg. The fatty acid composition is predominant by linoleic acid 65.7%, polyunsaturated fatty acid 66.03% and saturated fatty acids 17.87%.

[Read full article] [Citation]

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Elena UNGUREANU, Gabriel MUSTATEA, Mona Elena POPA

Food contact materials represent a possible source of contamination of the packaged products, through monomers or additives that can migrate from the packaging, especially the plastic ones, into the product. This type of contamination is an important problem related to the safety of food products, carrying out a lot of research in this regard. These chemical contaminants include Bisphenol A, with the highest volume produced worldwide, which is used as a plasticizer or intermediate product in obtaining polymeric materials, especially in polycarbonate material, but also in obtaining epoxy resins used for inner surface coatings of cans. The release of this compound is influenced by certain factors, among which the most important are the following: temperature, contact time and light exposure. The aim of this research article is to determine the levels of bisphenol A in different packed food and to verify compliance with the limit established by the legislation in force.

[Read full article] [Citation]

Page 5 of 12

The publisher is not responsible for the opinions published in the Volume. They represent the authors’ point of view.

© 2019 Scientific Bulletin Series F.“Biotechnologies“. All Rights Reserved. To be cited: Scientific Bulletin Series F.“Biotechnologies“.

Powered by INTELIDEV

Biotechnologies Journal