Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Amalia Carmen MITELUŢ, Georgiana-Aurora ŞTEFĂNOIU, Elisabeta Elena POPA, Paul Alexandru POPESCU, Mona Elena POPA

Food spoilage may be defined as a process or few changes that could lead to an undesirable or unacceptable product for consumption. Food stability may be hampered by the contamination of a large diversity of microbial spoilers, from prokaryotes (Gram-negative and Gram-positive bacteria) to unicellular (yeasts) and multicellular eukaryotes (moulds). Effective preventive measures and innovative preservation methods have been intensively studied with the aim of food spoilage reduction and food shelf life improvement. The primary focus of food preservation has been on controlling microbial populations, with a specific emphasis on pathogenic microorganisms. In the last years, innovative processes and technologies have been applied in food industry as non-conventional preservative hurdles, which also respond to consumer demand for safe minimally processed food. This paper studies the effect of radiofrequency (RF) treatment on food spoilage microorganisms.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Umesh MADAR, Devarajan THANGADURAI, Suyamindra KULKARNI, Pramod GAI, Jeyabalan SANGEETHA

Isolation of pure DNA is a significant component for PCR amplification and DNA fingerprinting analysis of plant species. Leaves of wild palms are generally hard, enormously fibrous, and are difficult to grind. The 25 species belong to 9 genera such as Areca (one species), Arenga (one species), Bentickia (one species), Borassus (one species), Calamus (fifteen species), Caryota (one species), Corypha (one species), Phoenix (three species) and Pinanga (one species) of the family Arecaceae and were collected from various parts of Southern India. An experiment was done to isolate the high molecular weight DNA from fibrous young leaves of twenty five wild palm species followed by several modifications in the novel CTAB DNA isolation method. DNA was isolated with slight modification in the CTAB method with liquid nitrogen and the quantification of obtained DNA was measured using a spectrophotometer and 0.8% agarose gel electrophoresis. DNA was further diluted with T10E1 buffer and optimized for ISSR-PCR amplification. Hence, the described protocol has proven to be advantageous due to its simple, efficient, affordable reagents resulting in a high molecular weight DNA of good quality from leaf fibrous tissues.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Thomas VEZETEU, Adriana AURORI, Daniel DEZMIREAN, Adriana CRISTE

The European honey bee, Apis mellifera, as most insects of the world, is currently facing major difficulties and, particularly for honey bees, this results in significant colony losses. One of the most stressful factors for A. mellifera is the ectoparasitic mite, Varroa destructor. V. destructor invasions are largely treatable and preventable, however they bring forth great challenges to A. mellifera populations and breeders, making apiculture increasingly time and resource consuming. The global research in apiculture pathology is mostly focused on Varroa sp. This review will be focusing on the recent literature in Varroa treatment, prevention and parasite - host interaction.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Maria GEORGIEVA

The aim of the present study is to observe the capacity of adventitious organogenesis of leaves and stem segments of six cultivars of highbush blueberry. The effect of WPM cultural medium enriched with 3 mg/l zeatin, 3 mg/l zeatin ribozoid and 2 mg/l 2-iP on the regeneration potential, average and total regeneration rates was assessed. Direct organogenesis is different for individual genetic types of genus Vaccinium. The regenerative response in most of the variants is in favour of the stem segments as explant sources. The highest average number of regenerants was recorded in leaf segments of 'Bluejay' genotype - 5.6 numbers/explant at A_zr medium.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Alice GRIGORE, Svetlana COLCERU-MIHUL, Corina BUBUEANU, Lucia PIRVU, Roxana MATEI, Ionica BAJENARU, Ileana Paraschiv, Cornelia NICHITA

Plants are widely used for the development of new products for cosmetics and pharmaceutical applications. In recent years, it is increasingly recognized that the active properties of plant extracts, which are usually complex mixtures of many components, are not due to the activity of a single component, but is the result of the synergistic effects of the elements of the mixture. The aim of this study was to demonstrate the feasibility of a poly-herbal formulation based on Daucus carota L. and Prunus armeniaca L. to be included in cosmetics as an active ingredient. Three types of extracts were taken into consideration: hydroalcoholic, hydroglycerin and macerate in grapeseed oil. HPLC analysis revealed that the hydroalcoholic extract contains polyphenolcarboxylic acids rather than flavonoids. As regards fatty acids quantified by GC, slight differences between sample and control were recorded. The potential effect of the three extracts on murine fibroblasts was evaluated and the proliferation rate was assesed by MTS test. It was showed that none of the extracts are not exhibiting cytotoxic effects. Except for the maximum concentration tested of the hydroglycerin extract, at which the cell viability was around 50%, most of the samples exceeded the 80% threshold. In the case of the hydroglicerin extract, it was observed a strong stimulating effect on cell proliferation compared to control group. It can be concluded that this poly-herbal combination could be used successfully as cosmetic ingredient for its wound healing and regenerative properties.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Simona COPACI, Ştefana JURCOANE

Camelina (Camelina sativa) is a flower plant belonging to the family Brassicaceae, originating in Eastern Europe. Vegetable oil is not only for food use, it can also be used in many fields, such as the cosmetics industry. The oil obtained from Camelina seeds has a varied content of fatty acids with an intake of 50-60% unsaturated fatty acids, 35- 40% omega 3 content and 15-20% omega 6. It has a high content of omega 3, being one of the richest vegetable sources compared to this fatty acid. In this review, we discuss how some of the latest scientific advances such as the high content of tocopherol (E vitamin), the ability to extract lecithin from Camelina oil and the likelihood of being a replacement for the castor oil. Other approaches demonstrate the stability of Camelina oil both in the presence of synthetic and natural antioxidants. Finally, we discuss the potential of Camelina oil to be used in the cosmetics industry.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Mihaela MULȚESCU, Marta ZACHIA, Floarea BURNICHI, Florentina ISRAEL-ROMING

Brassica vegetables belong to the Cruciferous family and include different kinds of cabbage (white, red), cauliflower and broccoli. These vegetables are recognised for their contribution to human health and nutrition. Brassica vegetables are extensively studied, analysed and characterised lately due to their antioxidant character and antioxidant capacity. The aim of the study was to investigate the antioxidant capacity of four white cabbage varieties (ʻDe Buzăuʼ, ʻBuzoianaʼ, ʻMăguraʼ, ʻDe Işalniţaʼ). The content of total polyphenolics of fresh vegetables (Folin Ciocalteu procedure) was assessed as well as carotenoids and chlorophyll pigments (spectrophotometric methods) and vitamin C content (titrimetric assay using 2,6-dichlorphenol indophenol). The antioxidant activity of the vegetables was determined according to DPPH (2,2-diphenyl-1-picrylhydrazyl) protocol. The results showed the highest concentration of antioxidant compounds for Magura variety, while the lowest one was for De Isalnita variety. A study regarding stability of antioxidant capacity during storage for three months was performed too.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Luminiţa Raluca SIMIONESCU, Narcisa BĂBEANU, Călina Petruţa CORNEA

The paper aimed to present a review on the social and economic aspects of NBTs, the studies on two different species of plants, subjects of NBT's. The plants variants generated by NBTs are more readily accepted in the market and for crop improvement. In this article we will present briefly the benefits, application and expected developments, regulatory status of NBTs in and outside the EU. It was developed a system for the detection of a broad spectrum of GMOs for analysis of food/feed matrices by the characterization of transgene flanking regions and the typical combinations for transgene constructs. We will describe two different species of plants, subjects of NBTs: 1) Tomatoes for carotenoid sequestration mechanisms and the carotenoid biosynthesis. The carotenoid accumulation and changes in carotenoid profiles suggest that the plastid can adapt to changes in carotenoid content through plastid differentiation and preferential sequestration; 2) Edited maize genome by biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. As a conclusion, CRISPR/Cas9 is the most used technology for genome editing due to its simplicity and efficiency. In this article, we aim to highlight the application of CRISPR/Cas9 technique system, like the powerful genome editing tool for crop improvement.

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A REVIEW OF IN VITRO STUDIES ON Allium tuncelianum (Kollman) Ozhatay, Matthew, Siraneci

Written by Faika YARALI KARAKAN
Pages 11 to 16

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BIOTECHNOLOGICAL RECYCLING OF FRUIT TREE WASTES THROUGH ORGANIC CULTIVATION OF MUSHROOM SPECIES

Written by Gabriela ȚEȚU
Pages 17 to 22

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PRELIMINARY RESULTS REGARDING THE TESTING OF TREATMENTS WITH LED ON THE SEED GERMINATION OF Lycopersicum esculentum L.

Written by Oana LIVADARIU, Ruxandra-Daniela DUMITRU
Pages 23 to 28

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COMPARATIVE ANALYSES OF PLANT RESPONSES TO SALINITY IN RELATED TAXA: A USEFUL APPROACH TO STUDY SALT STRESS TOLERANCE MECHANISMS

Written by Oscar VICENTE, Monica BOSCAIU
Pages 29 to 36

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PHYLOGENETIC ANALYSES OF SUGARCANE CULTIVARS USING SIMPLE SEQUENCE REPEAT MARKERS

Written by Rakesh TAWADARE, Devarajan THANGADURAI, Rayappa KHANDAGAVE, Jeyabalan SANGEETHA, Ramachandra PANDHARI
Pages 37 to 41

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RAPID DNA ISOLATION AND ISSR-PCR OPTIMIZATION FOR FIBROUS LEAF TISSUES OF WILD PALMS OF SOUTHERN PENINSULAR INDIA

Written by Umesh MADAR, Devarajan THANGADURAI, Suyamindra KULKARNI, Pramod GAI, Jeyabalan SANGEETHA
Pages 42 to 48

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MOLECULAR DIVERSITY IN WILD POPULATIONS OF Pinanga dicksonii (Roxb.) Blume (Arecaceae) FROM WESTERN GHATS OF KARNATAKA USING MICROSATELLITE MARKERS

Written by Umesh MADAR, Devarajan THANGADURAI, Kusum Pramod ANGADI, Vadakkethil Balakrishnan SREEKUMAR, Jeyabalan SANGEETHA
Pages 49 to 56

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IDENTIFICATION OF A SMALL HEAT SHOCK PROTEIN GENE FROM PISTACHIO

Written by Mehmet KOC, Muhittin KULAK, Muhammet KARAMAN, Hakan ÇETINKAYA
Pages 57 to 62

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RECENT DISCOVERIES IN Varroa destructor TREATMENT, PREVENTION AND PARASITE - HOST INTERACTION

Written by Thomas VEZETEU, Adriana AURORI, Daniel DEZMIREAN, Adriana CRISTE
Pages 63 to 70

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COMPARATIVE STUDY ON EXTRACTION METHODS OF PECTIN FROM BY-PRODUCTS OF JUICED CARROTS

Written by Agnes TOMA, Oana CRĂCIUNESCU, Rodica TATIA
Pages 71 to 77

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EFFECT OF CLIMATE CONDITIONS ON THE RIPENESS QUALITY INDICATORS OF SOME RED GRAPES FROM VALEA CĂLUGĂREASCĂ VINEYARD

Written by Elena COARFĂ, Mona Elena POPA
Pages 78 to 86

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IN VITRO RESEARCH ON THE INHIBITORY EFFECTS OF FENNEL, SAGE AND SEABUCKTHORN ESSENTIAL OILS ON SOME FOOD SPOILAGE FUNGI

Written by Georgia OLARU, Elena Mona POPA
Pages 87 to 90

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HEAVY METALS AND PHYSICO-CHEMICAL COMPOSITION OF MATERNAL BREAST MILK AND COLOSTRUM

Written by Igori BALTA, Dragoș OLA, Roxana FILIP, Ana Maria PUI, Renata SALANTAI, Mihai COTÎRȚĂ, Ioan ȚÂNTEA, Adina Lia LONGODOR, Zamfir MARCHIŞ, Aurelia COROIAN
Pages 91 to 101

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APPLICATION OF HURDLE TECHNOLOGY AS A NOVEL APPROACH TO NEW DIETARY FIG-BASED PRODUCTS DEVELOPMENT IN RURAL AREAS OF ALBANIA

Written by Luziana HOXHA, Renata KONGOLI
Pages 102 to 107

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RESEARCHES REGARDING THE ANTIOXIDANT POTENTIAL OF SELECTED Brassicaceae VEGETABLES REPRESENTATIVE FOR HUMAN NUTRITION

Written by Mihaela MULȚESCU, Marta ZACHIA, Floarea BURNICHI, Florentina ISRAEL-ROMING
Pages 108 to 116

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ISOLATION AND IDENTIFICATION OF EFFECTIVE MICROBIAL STRAIN FOR ACCELERATED BIODEGRADATION OF LEATHER INDUSTRY WASTE

Written by Mioara Ancuța DUMITRU, Ștefana JURCOANE, Daniela BĂLAN, Oana SICUIA
Pages 117 to 121

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EFFECT OF ACUTE GAMMA IRRADIATION ON GENERATION TIME, LIPID, CHLROPHYLL A AND CAROTENS, IN Chlorella sorokiniana UTEX 2130 AND Synechocystis PCC 6803

Written by Cristina MOISESCU, Ana-Valentina ARDELEAN, Daniel NEGUT, Ioan I. ARDELEAN
Pages 122 to 127

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LACCASE: MACRO AND MICROBIAL SOURCES, PRODUCTION, PURIFICATION AND BIOTECHNOLOGICAL APPLICATIONS

Written by Cristina Valentina ALBU (PROCA), Raluca Ştefania ENCEA, Camelia DIGUŢĂ, Florentina MATEI, Călina Petruţa CORNEA
Pages 128 to 136

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TRENDS ON PHARMACEUTICAL PACKAGING MATERIALS

Written by Denisa CIOTEA, Mona Elena POPA
Pages 137 to 142

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HIGHER UTILIZATION OF WASTE FROM THE FOOD INDUSTRY THROUGH BIOTECHNOLOGICAL METHODS

Written by Diana GROPOŞILĂ-CONSTANTINESCU, Luminiţa VIŞAN, Gabriela MĂRGĂRIT, Radu-Cristian TOMA, Dana BARBA, Marius HANGAN
Pages 143 to 146

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EFFICIENT UTILIZATION OF WATERMELON WASTES

Written by Indira GALIT, Alexandru Cristian GROSU, Narcisa BĂBEANU, Ovidiu POPA
Pages 147 to 153

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MAPPING GEOGRAPHICAL DISTRIBUTION OF ROMANIAN ORCHIDS AND THEIR BIOLOGICAL ACTIVE SUBSTANCES

Written by Laura-Simona IRIMESCU, Narcisa BĂBEANU, Florentina MATEI
Pages 154 to 163

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PRELIMINARY CHARACTERIZATION IN VITRO OF Bacillus licheniformis STRAIN FOR USED AS DIETARY PROBIOTIC

Written by Mihaela DUMITRU, Ionuț SORESCU, Mihaela HĂBEANU, Cristina TABUC, Ştefana JURCOANE
Pages 164 to 172

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RESEARCHES CONCERNING THE USE OF CAMELINA OIL IN THE COMPOSITION OF COSMETIC PRODUCTS: REVIEW

Written by Simona COPACI, Ştefana JURCOANE
Pages 173 to 180

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ANTIOXIDANT CAPACITY IN DONKEY MILK (Equus asinus) DEPENDING ON LACTATION

Written by Adina Lia LONGODOR, Vioara MIREŞAN, Răzvan A. CODEA, Camelia RĂDUCU, Luisa ANDRONIE, Zamfir MARCHIȘ, Igori BALTA, Codruţa MARIŞ, Aurelia COROIAN
Pages 181 to 185

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PHYSICO-CHEMICAL COMPOSITION AND ANTIOXIDANT CAPACITY OF BUFFALO MILK

Written by Aurelia COROIAN, Camelia RĂDUCU, Vioara MIREŞAN, Daniel COCAN, Igori BALTA, Adina Lia LONGODOR, Luisa ANDRONIE, Mircea MUNTEAN, Zamfir MARCHIŞ
Pages 186 to 192

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CHARACTERIZATION OF MERLOT DRY RED WINE COMPOSITION ACCORDING TO THE YEAR OF PRODUCTION

Written by Camelia RĂDUCU, Vioara MIREȘAN, Igori BALTA, Adina Lia LONGODOR, Ștefania MARIS, Aurelia COROIAN
Pages 193 to 198

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AN OVERVIEW ON HUMAN POTENTIAL EXPOSURE TO BISPHENOL A FROM FOOD-CONTACT MATERIALS USED IN FRUITS PACKAGING AND PROCESSING

Written by Elena UNGUREANU, Gabriel MUSTĂȚEA, Mona Elena POPA
Pages 199 to 204

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TRENDS IN REFRIGERATION TECHNOLOGIES USED FOR FOOD PRESERVATION – A REVIEW

Written by Elisabeta Elena POPA, Amalia Carmen MITELUȚ, Mona Elena POPA
Pages 205 to 210

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THE MOUNTAIN PRODUCT - THE VISIT CARD OF THE MOUNTAIN AREAS

Written by Ioana TOMA, Florentina MARIN HADDAD, Cristina GARLEA, Mariana PIPIRIGEANU, Mona Elena POPA
Pages 211 to 215

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REVIEW ON LEGAL, SOCIAL AND ECONOMIC ASPECTS OF THE NEW BREEDING TECHNIQUES

Written by Luminiţa Raluca SIMIONESCU, Narcisa BĂBEANU, Călina Petruţa CORNEA
Pages 216 to 225

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INFLUENCE OF LACTATION ON COMPOSITION OF MARE'S MILK

Written by Zamfir MARCHIȘ, Adina Lia LONGODOR, Răzvan A. CODEA, Igori BALTA, Aurelia COROIAN
Pages 226 to 229

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THE NUTRITIONAL AND LIFESTYLE HABITS OF THE STUDENTS' POPULATION AT THE UNIVERSITY OF BIHAĆ AND PRESENTS THE RISK FACTORS FOR COLORECTAL CANCER

Written by Suzana JAHIĆ, Jasmina CEPIĆ, Emina MALKIĆ
Pages 230 to 236

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DEBUNKING MISINFORMATION ABOUT FOOD

Written by Huub LELIEVELD, Veslemøy ANDERSEN
Pages 237 to 250

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CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF Hyssopus officinalis L. SELECTIVE FRACTIONS OBTAINED BY DIFFERENT METHODS

Written by Alice GRIGORE, Svetlana COLCERU-MIHUL, Corina BUBUEANU, Lucia PÎRVU, Cristina BAZDOACĂ, Sultana NIŢĂ
Pages 251 to 255

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COLOUR AND ORIGIN OF POLLEN PELLETS FROM TWO FRESH BEE POLLEN SAMPLES – A PRELIMINARY ANALYSIS

Written by Monica ENACHE, Larisa ANGHEL
Pages 256 to 259

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INITIAL STEPS TOWARDS THE ESTABLISHMENT OF A POLLEN COLLECTION AT USAMV BUCHAREST: THE STUDY OF ALLERGENIC POLLEN

Written by Monica ENACHE, Matei COMAN, Marius HANGAN
Pages 260 to 265

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Daniel NIŢOI, Camelia Filofteia DIGUŢĂ, Florentina MATEI, Călina Petruţa CORNEA

Candida, known as an “opportunistic” human pathogen, has coevolved with humans by persisting in mucosa and on the skin. The mechanism and factors determining the transformation of the “conolization” status into disease status is still under research. However, new preventive solutions for such events are under investigation. In our study we have tried to prove that some lactic acid bacteria (LAB), isolated from natural fermented food sources (Kombucha and fermented cocoa beans) have inhibitory effect on different species of Candida with different pathogenic potential (C. albicans, C. parapsilosis, C. krusei and C. guilliermondii). The inhibitory tests employed two different methods (transversal and radial strike). Among nine LAB strains isolated from cocoa beans fermentation, two have significant inhibitory activity against Candida parapsilosis. In the case of Kombucha source, two out of seven LAB strains have significant inhibitory activity against Candida albicans, Candida krusei and Candida parapsilosis; low level inhibition has been noticed against Candida guilliermondii. When mixing different combination of LAB from both natural sources, the anti-Candida effect was neither augmented, nor decreased.

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