ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364
 

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Aurelia COROIAN, Camelia RĂDUCU, Vioara MIREŞAN, Daniel COCAN, Igori BALTA, Adina Lia LONGODOR, Luisa ANDRONIE, Mircea MUNTEAN, Zamfir MARCHIŞ

Buffalo milk due to its high fat content from the energy point of view is more valuable compared to cow's milk. The percentage of fat varies depending on the stage of lactation, season, individual, health, animal age and diet. The study of the chemical composition and the antioxidant capacity of buffalo milk is required for both scientific and technological considerations, given the importance of this type of milk in the consumer's diet. The purpose of this study was to evaluate the antioxidant capacity and the physico-chemical composition of buffalo milk by lactation. Buffalo milk can have different qualities, so the quality of buffalo milk is also determined by its content in its components (protein, fat, lactose, vitamins, fatty acids, water content, antioxidant capacity). The physicochemical parameters and antioxidant capacity were influenced by lactation, presenting the highest values in lactations III and IV. During lactation I, parameters such as fat, protein and lactose showed a content of 7.88, 4.35 and 4.71%, respectively. Furthermore, in lactation IV, fat, protein and lactose had increased, showing the content of these parameters as follows 9.53, 4.68 and 4.77%. Antioxidant capacity of buffalo milk showed the highest numbers in lactation III (360.1) and IV, 358.9 μg/ml. From all of the analyzed parameters, only total dry substance content presented the most increased values 18.9% in lactation I.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Yuriy TCHOURSINOV, Olena KOVALIOVA, Viktoriia KALYNA, Svitlana MYKOLENKO, Nadiia KHOMUK

The results of the qualitative assessment of the final products of the technology include: mowing of vegetative plants, such as alfalfa, amaranth, clover, pea-oat mixture; grinding; wet fractionation with obtaining marc and juice; thermal coagulation, filtration of juice, drying of the chloroplast fraction by spraying or in a vibro-boiling layer.In the process of transformation of vegetative plants, biologically active phyto-feed additives are obtained as the final product. During the production of which the processed phytomaterials were influenced by regime processing parameters related to temperature, processing of time on certain stages, acidity, etc. A computational method developed in the paper for determining the biological value of phyto-feed additives is implemented taking into account the importance of protein efficacy ratio.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Cristina Monica DABULICI, Emanuel VAMANU

For skin care, the organic ingredients have been used by folk products. Today, they are becoming more used because of the toxic action of chemical products. Thus, there is a great demand that natural products should be used in cosmetic products. The main benefits that are determined by the plant extracts, used for skin care, contains antioxidant and anti-germ activities or the effect of halting of tyrosinase. Phenol compounds are the main group of natural antioxidants popular on the market and express anti-germ actions, anti-inflammatory or anti-aging, that can get pass the skin barrier. The purpose of the paper was to present a revised and detailed list of the main factors that have effects on the skin, a detailed list of benefits, from a scientific point of view, of topic anti-oxidants, to understand the way of formulating and administering of topic anti-oxidants protection study.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXV, Issue 1
Written by Orsolya Csilla RADULY, József FAZAKAS, Diana CONSTANTINESCU-ARUXANDEI, Csilla BENEDEK, Mălina AVRAM-DEȘLIU, Enikö FAZAKAS, Florin OANCEA

Porous ceramic granules (PCGs) present an interconnected pores architecture, which mimics the spatial arrangement of the pore spaces and of the solid particles, mineral and/or organic, in the soil. More than 2/3 of the volumes of the ceramic granules used as soil improvers are pores of different sizes – macro, micro and nanopores. Around one half of these pores are capillary pores, which retain and slowly release water and mineral nutrients. The other half of the pores are non-capillary pores, retaining gases and supporting soil aeration. This PCGs porous structure, related to the efficiency as soil improver, is highly compaction-resistant, making PCGs an effective soil conditioner for cultivated plants that support high traffic, such as turf and ornamental garden plants. This paper reviews the use of porous ceramic granules as inorganic soil improver for horticultural plants.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Mihaela DUMITRU, Ionuț SORESCU, Mihaela HĂBEANU, Cristina TABUC, Ştefana JURCOANE

The purpose of this paper was to provide a direction for evaluating the probiotic potential and safety of a microbial strain in order to use in weaning piglets. Bacillus licheniformis ATCC 21424 was analyzed morphologically, culturally, biochemically, for hemolytic activity and enzymatically (amylase and protease screening). In vitro, some probiotic properties were study as: resistance to pH by simulated gastric juice (pH 2 and 3) and bile salts (simulated intestinal fluid). The biochemical characteristics was performed by catalase assay, API 50 CHB Biomerieux strips, apiweb API 50 CHB V 4.0 soft (B. licheniformis, % ID 99.9) and ABIS online (~90.6% similarity). The hemolytic activity was assayed on blood agar medium. The strain was grown in nutrient medium, in two ways: static incubation (37C, 24 h, 4 x 108 CFU/ml) and under constant agitation (37C, 24 h, 120 rpm, 1.56 x 1010 CFU/ml). To screen out, the most favorable carbon source was included in the basal medium (1% w/v, pH = 7) glucose (12.11 ± 0.1), fructose (11.73 ± 0.67), lactose (12.03 ± 0.14) and starch (12.51 ± 0.27). The strain is a Gram-positive, rod-shaped bacteria, arranged in short chains or in small irregular pairs with ability to produce spores. The endospores were central, paracentral and subterminal. The strain growth was aerobic and was non – hemolytic. The enzymatic process was observed by appearance of distinct zones around strain colonies. The strain presents relatively good viability at pH 3 and tolerated oxgall (0.3%). In conclusion, the results suggested that the strain present some probiotic traits and can be further assessed for other probiotic characters as antibacterial activity, induction of local immune response etc.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Getuța DOPCEA, Alina Elena NANU, Ioan DOPCEA, Călina Petruţa CORNEA, Camelia Filofteia DIGUȚĂ, Florentina MATEI

Povidone iodine (PVP-I) is a valuable, widespread antiseptic that could trigger bacterial cross-resistance to antibiotics when used at sub-lethal concentrations, although no acquired bacterial cross-resistance has been reported for PVP-I. In this study are assessed the changes in the antimicrobial susceptibility profile of Staphylococcus aureus and Staphylococcus epidermidis strains after five exposures to 0.3125 % PVP-I. For all Staphylococcus epidermidis tested strains the mean of the minimum inhibitory concentrations (MICs) and of the minimum bactericidal concentrations (MBCs) significantly decreased after repeated exposures to sub-lethal concentrations of PVP-I compared to the initial values. There was no significant change from baseline in mean results of the disk diffusion test of any antibiotic for the tests performed on the analysed strain.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIII
Written by Oana LIVADARIU, Ruxandra-Daniela DUMITRU

The present study discusses the preliminary results obtained from testing the influence of LED treatments over seed germination of three different Lycopersicum esculentum L. varieties. The respective varieties have been chosen for their determined growth pattern, which would make them suitable for implementation within a vertical farming system. Speeding up the germination process and ensuring that most, if not all the seeds germinate is relevant in obtaining healthy and productive L. esculentum L. crops suitable for indoor farming, which will provide a sustainable solution for a round-the-year constant production which can meet the market request, no matter the climatic conditions specific to the area. The experimental results obtained show that treatments with light emitted of LED (white, blue, red and natural light), on the seed germination of Lycopersicum esculentum L. varieties (Buzău 4C, Rio Grande ST and Saint Pierre ST), may influences: the sprouts rate, the fresh weight of sprouts, the fresh weight of cotyledons, the fresh weight of hypocotyls, the fresh weight of roots, the length of hypocotyls and length of roots.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV, Issue 2
Written by Laura-Simona IRIMESCU, Camelia Filofteia DIGUŢĂ, Raluca Ştefania ENCEA, Florentina MATEI

This paper aims to provide preliminary data about the antimicrobial potential of an ornamental flower, respectively Phalaenopsis orchid. Products from different parts of the orchids, like roots, leaves or flowers, are already in use for different purposes. The orchid plants used in the study were declared florists’ waste, and they were carried out tests with methanolic extracts made of different parts of these orchids (roots, leaves, stems). Using the spot technique, it was tested the inhibitory activity of different methanolic extracts on potential pathogens, respectively four Candida spp and on four different Staphylococcus spp. Due to their high diversity, the bioactive compounds from plants are of great interest results have shown promising inhibitory activity of orchid extracts against Candida krusei.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXV, Issue 1
Written by Eleonora CALZONI, Alessio CESARETTI, Nicolò MONTEGIOVE, Roberto Maria PELLEGRINO, Leonardo LEONARDI, Carla EMILIANI

The degradation of biomasses derived from agriculture and food industry presents the double advantage of both eliminating often polluting wastes and giving the possibility of introducing novel bio-derived products into the market. The recovery of the protein component and its transformation into protein hydrolysates is generally carried out chemically or enzymatically and brings great added value to waste biomasses. The recovery of the protein component from waste products is extremely advantageous inasmuch as bio-based products can be reintroduced into the market by virtue of their high added value in full compliance with the perspective of the Circular Economy. The products obtained can be used as food supplements both in human and animal nutrition, as growth stimulants in agriculture, or as biofertilizers. The peculiar characteristics of the obtained protein hydrolysates allow their multiple applications in many industrial fields. Therefore, the aim of this work is to assess the protein and amino acid (AA) profiles of agri-food biomass, i.e. soy wastes, previously subjected to an enzymatic hydrolysis process. The protein profile of the initial biomass and the corresponding hydrolysate was analyzed by SDS-PAGE followed by Coomassie Blue staining, while the AA profile was evaluated by quadrupole time-of-flight liquid chromatography/mass spectrometry (Q-TOF LC/MS). The results indicate how the enzymatic hydrolysis process breaks down the peptide bonds of the protein component in the biomass, leading to the formation of hydrolysates rich in small peptides and free AAs.

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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Ana-Simona COPACI, Ştefana JURCOANE

Camelina oil is obtained by pressing the seeds of Camelina sativa. Their pressing is done by a cold process. The oil samples used were obtained from the Camelina sativa - Mădălina variety, which was cultivated at the Belciugatele farm, with a working point Moara Domneasca (Găneasa commune, Ilfov county) between 2017-2018. The present project aims at the use of camelina oil in cosmetic preparations, using 4 types of oil grown in an ecological system, but their storage was different namely: some of the samples were kept at ambient temperature, in plastic dark blue cans, their capacity being 10l, but they were filled with 5l oil, in parallel being kept experiments at the refrigerator in brown glass containers, fully filled, in order to obtain the purest oil, but also the most complete one in terms of preservation of the biochemical qualities. The determinations made consisted of: appearance, odor, color, determination of iodine and saponification indices, as well as composition in fatty acids. All these determinations have led to the most efficient flow of camelina oil purification used in the cosmetic industry.

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